Analysis of K-Ras and p53 Mutations in Mesotheliomas from Humans and Rats Exposed to Asbestos

Details

• Personal Author:
  Keshava N ; Liu YQ ; Ni Z ; Ong T ; Whong, Wen-Zong ; Zhou G
• Description:
  Malignant mesothelioma is known to be associated with asbestos exposure. However, the mechanism of mesothelial carcinogenesis in relation to the activation of proto-oncogenes or inactivation of tumor suppressor genes remains unclear. In this study, the PCR-Primer Introduced Restriction Site (PCR-PIRS) assay was employed to examine mutations in the K-ras proto-oncogene in mesothelioma tissues from workers exposed to asbestos and from rats treated with asbestos. Mutations in exons 5-8 of the p53 tumor suppressor gene were determined by direct DNA sequence analysis. Results of the PCR-PIRS analysis revealed no mutations in codons 12, 13 or 61 of the K-ras gene in any of the 17 human or 22 rat mesothelioma tissue samples. These results were confirmed by direct DNA sequence analysis. No mutations were found in exons 5-8 of the p53 gene in any of the mesothelioma tissue samples analyzed. These results and the results reported by others indicate that the K-ras proto-oncogene and p53 tumor suppressor gene may not play a critical role in the induction of mesothelioma by asbestos either in humans or in rats. [Description provided by NIOSH]
• Subjects:
  Animals Asbestos Carcinogens Environmental Exposure Genetics Inorganic Chemicals Laboratories Neoplasms Occupational Health Safety
• Keywords:
  Animal Studies Asbestosis Author Keywords: Asbestos Carcinogenesis Carcinogenicity Exposure Assessment Exposure Levels Gene Mutation Genes K-ras Proto-oncogene Laboratory Animals Mesothelial Cells Mesotheliomas Oncogenesis Oncogenicity P53 Tumor Suppressor Gene Tumors

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• ISSN:
  0027-5107
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  87-92
• Volume:
  468
• Issue:
  1
• NIOSHTIC Number:
  nn:20020766
• Citation:
  Mutat Res 2000 Jun; 468(1):87-92
• Contact Point Address:
  Toxicology and Molecular Biology Branch, National Institute for Occupational Safety and Health, HELD, NIOSH, m/s 3014, 1095 Willowdale Road, Morgantown, WV 26505-2888, USA
• Email:
  too2@cdc.gov
• CAS Registry Number:
  Asbestos (CAS RN 1332-21-4)
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  True
• Source Full Name:
  Mutation Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f802d4e2b4dd48eaa3c0a0ccc2a38e4a20dd41bbcdaf59eb10bc3d054adbed8128eb621d7651855304cd9186cedb0df07e609fac96698fd20c0ad105f483d45b
• Download URL:
  https://stacks.cdc.gov/view/cdc/198434/cdc_198434_DS1.pdf
• File Type:
  [PDF - 78.50 KB ]