Analysis of K-Ras and p53 Mutations in Mesotheliomas from Humans and Rats Exposed to Asbestos

Details

• Personal Author:
  Keshava N ; Liu YQ ; Ni Z ; Ong T ; Zhou G
• Description:
  Malignant mesothelioma is known to be associated with asbestos exposure. However, the mechanism of mesothelial carcinogenesis in relation to the activation of proto-oncogenes or inactivation of tumor suppressor genes remains unclear. In this study, the PCR-Primer Introduced Restriction Site (PCR-PIRS) assay was employed to examine mutations in the K-ras proto-oncogene in mesothelioma tissues from workers exposed to asbestos and from rats treated with asbestos. Mutations in exons 5-8 of the p53 tumor suppressor gene were determined by direct DNA sequence analysis. Results of the PCR-PIRS analysis revealed no mutations in codons 12, 13 or 61 of the K-ras gene in any of the 17 human or 22 rat mesothelioma tissue samples. These results were confirmed by direct DNA sequence analysis. No mutations were found in exons 5-8 of the p53 gene in any of the mesothelioma tissue samples analyzed. These results and the results reported by others indicate that the K-ras proto-oncogene and p53 tumor suppressor gene may not play a critical role in the induction of mesothelioma by asbestos either in humans or in rats. [Description provided by NIOSH]
• Subjects:
  Animals Asbestos Carcinogens Environmental Monitoring Genetics Inorganic Chemicals Laboratories Neoplasms Occupational Health Safety
• Keywords:
  Animal-studies Asbestosis Carcinogenesis Carcinogenicity Exposure-assessment Exposure-levels Gene-mutation Genes Laboratory-animals Mesothelial-cells Oncogenesis Oncogenicity Tumors

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• ISSN:
  0893-6692
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  44
• Volume:
  35
• NIOSHTIC Number:
  nn:20026120
• Citation:
  Environ Mol Mutagen 2000 Apr; 35(Suppl 31):44
• CAS Registry Number:
  Asbestos (CAS RN 1332-21-4)
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  False
• Source Full Name:
  Environmental and Molecular Mutagenesis
• Supplement:
  31
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7d30b26f14649929b2c5b74d508707a5e57f1cdc5cd93719b35ad9495c382324955a71e85a0e8b9d080202352c4c96e653d3e30c93a50ec86515110b96638ced
• Download URL:
  https://stacks.cdc.gov/view/cdc/197196/cdc_197196_DS1.pdf
• File Type:
  [PDF - 208.43 KB ]