Effects of Metal Working Fluids on B6C3F1 Mouse Skin

Details

• Personal Author:
  Al-Humadi NH ; Battelli L ; Castranova, Vincent ; Kommineni C ; Schwegler-Berry D ; Willard PA
• Description:
  Over 10 million workers in the United States are exposed to metal working fluids (MWFs) through dermal contact and/or inhalation of aerosolized fluids. The objective of this study was to elucidate the response of skin to dermal exposure to MWFs. Four- to six-week-old B6C3F1 mice of both sexes were divided into eight groups (n=5/group) and exposed to 200 l of 0%, 5% (pH 7 and 9.7) and 100% (pH 10.4), unused MWFs/H2O by topical application to the unshaven back (cervical to sacral region), twice a week for 6 weeks. Skin-mast cell number in females of most treated groups and of two male groups (100% and 5%, pH 7) were significantly higher than the control groups. Eventhough both males and females (treated with 100% MWF/H2O) showed an increase in the skin-histamine levels (38% and 41%, respectively), this increase was significant in females only (ANOVA, P0.05). Dermal exposure to 100% MWFs increased liver weight significantly in both sexes. Ulcers and associated inflammation were seen in the skin of mice treated with 100% unused MWFs and sacrificed at 6 weeks, but not in the recovery groups. Hypertrophy of the sebaceous gland epithelium is present in all mice treated for 6 weeks and sacrificed immediately. However, only the mice treated with 100% MWF retained the hypertrophy of this epithelium after a 6-week recovery period. In conclusion, dermal exposure to unused semi-synthetic MWF penetrates the normal skin, induces mast cell accumulation in the skin, produces hypertrophy of the sebaceous glands, and may affect females more than males. [Description provided by NIOSH]
• Subjects:
  Aerosols Animals Demography Digestive System Digestive System Diseases Laboratories Liver Metals Metal Workers Occupational Exposure Occupational Health Respiratory System Safety Sex Characteristics Skin

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• Keywords:
  Animal Studies Author Keywords: Histamine Demographic Characteristics Inhalation Studies Kidney Laboratory Animals Liver Liver Disorders Mast Cells Metalworking Metal Working Fluid Metalworking Fluids Metalworking Industry Mice Occupational Exposure Sex Factors Skin Skin Exposure Ulcer Workers

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• ISSN:
  0748-2337
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  203-210
• Volume:
  16
• Issue:
  6
• NIOSHTIC Number:
  nn:20020603
• Citation:
  Toxicol Ind Health 2000 Jul; 16(6):203-210
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology and Industrial Health
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0dba25256d5a63b50b2246db7700fced00b7ba1a66b72023c6580d692666ea35a3bb6cdc38666f420e7737f98b4c0829b51181b698670cf6d89de8eff20a8c96
• Download URL:
  https://stacks.cdc.gov/view/cdc/198303/cdc_198303_DS1.pdf
• File Type:
  [PDF - 13.81 MB ]