Elevated Oxidative Stress in Skin of B6C3F1 Mice Affects Dermal Exposure to Machine Working Fluid

Details

• Personal Author:
  Al-Humadi N ; Castranova, Vincent ; Kisin ER ; Kommineni CV ; Shvedova AA
• Description:
  Metal working fluids (MWFs) are widely used in industry for metal cutting, drilling, shaping, lubricating, and milling. Potential for dermal exposure to MWFs exists for a large number of men and women via aerosols and splashing during the machining operations. It has been reported earlier that occupational exposure to MWFs causes allergic and irritant contact dermatitis. Previously, we showed that dermal exposure of female and male B6C3F1 mice to 5% MWFs for 3 months resulted in accumulation of mast cells and elevation of histamine in the skin. Topical exposure to MWF also resulted in elevated oxidative stress in the liver of both sexes and the testes in males. The goal of this study was to evaluate the interaction between oxidative stress in the skin and topical application of MWF. Oxidative stress in skin of B6C3F1 mice of both sexes was generated by intradermal injection of the hydrogen peroxide (H2O2) -producing enzyme, glucose oxidase with polyethylene glycol (GOD+PEG). In mice given GOD+PEG, topical treatment with MWF (200 microl, 30%, for 1, 3, or 7 days) resulted in a mixed inflammatory cell response, accumulation of peroxidative products, and reduction of GSH content in the skin. Such changes were not observed with MWF treatment alone. These data indicate that oxidative stress can enhance dermal inflammation caused by occupational exposure to MWF. [Description provided by NIOSH]
• Subjects:
  Aerosols Animals Demography Dermatitis Environmental Exposure Hazardous Substances Hypersensitivity Laboratories Manufacturing And Industrial Facilities Metals Metal Workers Occupational Exposure Occupational Health Respiratory System Safety Sex Characteristics Skin Skin Diseases

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• Keywords:
  Allergic Dermatitis Animal Studies Author Keywords: Glucose Oxidase Contact Dermatitis Demographic Characteristics Exposure Levels GSH Health Hazards Inflammation Inhalation Studies Laboratory Animals Machine Operators Machinists Metalworking Metal Working Fluid Metalworking Fluids Metalworking Industry Occupational Exposure Oxidative Stress Sex Factors Skin Irritants Stress Workers

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• ISSN:
  0748-2337
• Publisher:
  Princeton Scientific Publishing Company Incorporated
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  267-276
• Volume:
  54
• Issue:
  1
• NIOSHTIC Number:
  nn:20020978
• Citation:
  Toxicol Ind Health 2000; 16(7-8):267-276
• Contact Point Address:
  Dr. Anna A. Shvedova, Health Effects Laboratory Division, Pathology and Physiology Research Branch, NIOSH m/s 2015, 1095 Willowdale Road, Morgantown , WV 26505
• Email:
  ats1@cdc.gov
• Editor(s):
  Mehlman MA
• Federal Fiscal Year:
  2000
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology and Industrial Health
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c0b73d7cf7f05becdbd9a38e28d382c719c935109e6377ca6f14f98299f279f4676577a3adba7feab60cbb4f73116edab2f89d37af02a6c0f03a30192cea7116
• Download URL:
  https://stacks.cdc.gov/view/cdc/198584/cdc_198584_DS1.pdf
• File Type:
  [PDF - 1.63 MB ]