Nitric Oxide Inhalation Transiently Elevates Pulmonary Levels of cGMP, iNOS mRNS, and TNF-Alpha

Details

• Personal Author:
  Brady TC ; Crapo J ; Mercer RR
• Description:
  The initial pulmonary vasodilation that occurs during nitric oxide. (NO) inhalation does not appear to be maintained chronically in many cases. NO may acutely relax vascular smooth muscle by increasing levels of guanosine 3',5'-cyclic monophosphate (cGMP), tumor necrosis factor (TNF) -alpha, and inducible nitric oxide synthase (iNOS) while decreasing levels of lipid peroxidation. It was hypothesized that the acute NO-induced changes in cGMP, TNF-alpha, iNOS, and lipid peroxidation, all of which may mediate vasodilation, are transient rather than sustained. Lungs from rats kept in chambers containing 6 parts/million. NO for 1 h, 1 day, or 1 wk were analyzed for levels of NO-induced vasodilatory mediators. Pulmonary cGMP, iNOS mRNA, and TNF-alpha were increased 1 h after NO exposure but decreased to control values at later times. Levels of malonyl dialdehyde, an indicator of lipid peroxidation, were decreased at all times during NO inhalation. As a whole, the data suggest that in lungs the vasodilatory mediators cGMP, iNOS, and TNF-alpha are only acutely and transiently elevated during inhalation of NO, consistent with the initially positive clinical response to inhaled NO that deteriorates over time. [Description provided by NIOSH]
• Subjects:
  Occupational Exposure Occupational Health Safety
• Keywords:
  Author Keywords: Lung Biological-models Chronic-exposure Critical Care Medicine Gas-inhalation Interferon-g Muscle-relaxation Pulmonary Hypertension Rats Superoxide Dismutase
• ISSN:
  1040-0605
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  275
• Issue:
  3
• NIOSHTIC Number:
  nn:20000649
• Citation:
  Am J Physiol Lung Cell Mol Physiol 1998 Sep; 275(3):L509-L515
• Contact Point Address:
  Todd C. Brady, Program in Integrated Toxicology, Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710
• CAS Registry Number:
  cGMP (CAS RN 7665-99-8) ; Interferon γ (human lymphocyte protein moiety reduced) (CAS RN 82115-62-6) ; Malonaldehyde (CAS RN 542-78-9) ; Nitric oxide (CAS RN 10102-43-9)
• Federal Fiscal Year:
  1998
• Peer Reviewed:
  True
• Source Full Name:
  American Journal of Physiology: Lung Cellular and Molecular Physiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5831a311bb1f18e19b8315efd98cc1297d78554a372561997e8f84699011f8181e9128dfdd80fe526a5b62ba08ed5e2853968201a3d241f68f4a235f9ebff021
• Download URL:
  https://stacks.cdc.gov/view/cdc/198183/cdc_198183_DS1.pdf
• File Type:
  [PDF - 160.50 KB ]