Aging-Dependent Regulation of Antioxidant Enzymes and Redox Status Inchronically Loaded Rat Dorsiflexor Muscles

Details

• Personal Author:
  Alway SE ; Baker BA ; Cutlip, Robert G. ; Docherty M ; Dudash HJ ; Geronilla KB ; Haff GG ; Ryan MJ
• Description:
  This study compares changes in the pro-oxidant production and buffering capacity in young and aged skeletal muscle after exposure to chronic repetitive loading (RL). The dorsiflexors from one limb of young and aged rats were loaded 3 times/week for 4.5 weeks using 80 maximal stretch-shortening contractions per session. RL increased H2O2 in tibialis anterior muscles of young and aged rats and decreased the ratio of reduced/oxidized glutathione and lipid peroxidation in aged but not young adult animals. Glutathione peroxidase (GPx) activity decreased whereas catalase activity increased with RL in muscles from young and aged rats. RL increased CuZn superoxide disumutase (SOD) and Mn SOD protein concentration and CuZn SOD activity in muscles from young but not aged animals. There were no changes in protein content for GPx-1 and catalase or messenger RNA for any of the enzymes studied. These data show that aging reduces the adaptive capacity of muscles to buffer increased pro-oxidants imposed by chronic RL. [Description provided by NIOSH]
• Subjects:
  Age Factors Animals Laboratories Musculoskeletal Diseases Musculoskeletal System Occupational Health Safety
• Keywords:
  Age-factors Animal-studies Author Keywords: Exercise Laboratory-animals Muscle-physiology Muscle-stress Musculoskeletal-system Oxidative Stress' Sarcopenia Superoxide Dismutase
• ISSN:
  0022-1422
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Issue:
  10
• NIOSHTIC Number:
  nn:20034724
• Citation:
  J Geront, Ser A Biol Sci Med Sci 2008 Oct; 63A(10):1015-1026
• Contact Point Address:
  Stephen E. Always, Laboratory of Muscle Biology and Sarcopenia, Division of Exercise Physiology, West Virginia University School of Medicine, Morgantown, WV 26506
• Email:
  salway@hsc.wvu.edu
• Federal Fiscal Year:
  2009
• Peer Reviewed:
  True
• Source Full Name:
  The Journals of Gerontology Series A: Biological Sciences and Medical Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:163f6d400b8748ce4297afcf34ee7d2d2321d6d673df49dd020e1627324ad4881d69c5afdd4f9656e87a486d6491071d2fe607e024dbc5d0d29b9a58c8188a24
• Download URL:
  https://stacks.cdc.gov/view/cdc/186826/cdc_186826_DS1.pdf
• File Type:
  [PDF - 1.13 MB ]