Nitric Oxide Alters Metabolism in Isolated Alveolar Type II Cells

Details

• Personal Author:
  Bowman L ; Huffman L ; Miles PR ; Bowman L ; Huffman L ; Miles PR
• Description:
  The effects of the nitric-oxide (10102439) generator S-nitroso-N- acetyl-penicillamine (SNAP) and the nitric-oxide-synthase (NOS) inhibitor NG-nitro-L-arginine-methyl-ester (L-NAME) on the synthesis of lung surfactant dipalmitoyl-phosphatidylcholine (DPPC), and some basic processes involved in energy metabolism were investigated. The study also examined the effects of some NOS inhibitors and external calcium on cellular nitric-oxide free radical production. Studies were conducted using alveolar type-II cells isolated from male Sprague-Dawley-rats. The findings demonstrate that the nitric- oxide (10102439) levels in alveolar type-II cells are able to affect lipid and energy metabolism. Exposure of the cells to nitric-oxide radicals from an external source causes reductions in disaturated phosphatidylcholines (DSPC) synthesis, cell ATP levels, and cellular oxygen consumption. However, inhibition of endogenous nitric-oxide radical levels induced by L-NAME, resulted in increased DSPC synthesis, cell ATP levels, and cellular oxygen consumption. It was also suggested that at least some of the endogenous nitric-oxide radicals may be generated by cNOS activity but that the precise regulatory role of NOS was not yet clear. [Description provided by NIOSH]
• Subjects:
  Energy Metabolism Enzymes Free Radicals Lung Occupational Health Respiratory System Respiratory Tract Diseases Safety Surface-Active Agents
• Keywords:
  Adenosine 5' Triphosphate Alveolar-cells Author Keywords: Lung Surfactant Enzyme-activity Free-radicals In-vitro-studies Lung-irritants Mammalian-cells Metabolic-study NIOSH-Author Respiratory-system-disorders Surfactants

  More +
• ISSN:
  1040-0605
• Document Type:
  Text
• Genre:
  Journal Article ; Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies ; DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  271
• Issue:
  1
• NIOSHTIC Number:
  nn:00236992
• Citation:
  Am J Physiol Lung Cell Mol Physiol 1996 Jul; 271(1):L23-L30
• Contact Point Address:
  P. R. Miles, Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown 26505
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9) ; Nitric oxide (CAS RN 10102-43-9)
• Federal Fiscal Year:
  1996
• Peer Reviewed:
  True
• Source Full Name:
  American Journal of Physiology: Lung Cellular and Molecular Physiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c70181f9b2b4535bf5eee7343f5fa70de1116636148d88ce7deecef3d4ca5594cfe42d5970a40cde98b2e98853e9386cc0de9605807710f225928feca72f299e
• Download URL:
  https://stacks.cdc.gov/view/cdc/197861/cdc_197861_DS1.pdf
• File Type:
  [PDF - 5.42 MB ]