Detection of DNA Adducts by Electron Capture Mass Spectrometry

Details

• Personal Author:
  Giese RW
• Description:
  Electron capture mass spectrometry (EC/MS) as a means of detecting DNA adducts was reviewed. The general concept of DNA adduction and its physiological significance were summarized. The general history of the development of EC/MS for detecting DNA adducts was outlined. Electrophores and their role in DNA adduct detection were discussed. The discussion considered the general concept of electrophores, conversion of DNA adducts into electrophores, electrophore subtypes, electrophore labels, the role of residual acidic hydrogen atoms in EC/MS analysis of DNA adducts, the types of derivatization reactions that can be used to convert DNA adducts into electrophores, the polar footprint concept as applied to EC/MS analysis of DNA adducts, and appropriate derivatization conditions for converting DNA adducts into electrophores. Specific applications of electrophore labeling for DNA adduct analysis by EC/MS were described. These include procedures for converting DNA adducts formed by 4-aminobiphenyl (92671), 4-aminofluorene (7083638), benzo(a)pyrene (50328), 4- (hydroxypyridyl)butanone, malondialdehyde (542789), 2- phosphoglycolate, and uracil (66228) into electrophores. Electrophores formed from compounds such as cytosine, methylcytosine, and hydroxymethyluracil were also described. The future prospects for EC/MS as a technique for determining DNA adducts were discussed. [Description provided by NIOSH]
• Subjects:
  Mass Spectrometry Occupational Health Safety
• Keywords:
  Analytical-methods DNA-adducts Grants-other Mass-spectrometry NIOSH-Contract NIOSH-Grant NIOSH-Publication Sample-preparation
• ISSN:
  0893-228X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Massachusetts ; OSHA Region 1
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  255-270
• Volume:
  10
• Issue:
  3
• NIOSHTIC Number:
  nn:00236384
• Citation:
  Chem Res Toxicol 1997 Mar; 10(3):255-270
• Contact Point Address:
  Medicinal Chemistry Northeastern University 360 Huntington Avenue Boston, MA 02115
• CAS Registry Number:
  4-Aminobiphenyl (CAS RN 92-67-1) ; 9H-Fluoren-4-amine (CAS RN 7083-63-8) ; Benzo[a]pyrene (CAS RN 50-32-8) ; Malonaldehyde (CAS RN 542-78-9) ; Uracil (CAS RN 66-22-8)
• Federal Fiscal Year:
  1997
• NORA Priority Area:
  Other Occupational Concerns ; Grants Other
• Performing Organization:
  Northeastern University, Boston, Massachusetts
• Peer Reviewed:
  True
• Start Date:
  19910101
• Source Full Name:
  Chemical Research in Toxicology
• End Date:
  19961130
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:94c316a407862c5579394b2eab4a5a771362d09f18a0f16de406327c90bb4a84a2d5c2483f1d9cd1e9b42ca41dbb99cbc390bc05dc6ff0ce56e780ef647a413f
• Download URL:
  https://stacks.cdc.gov/view/cdc/197830/cdc_197830_DS1.pdf
• File Type:
  [PDF - 416.72 KB ]