Characterization of Histamine Releasing Factors in Diisocyanate-Induced Occupational Asthma

Details

• Personal Author:
  Alam R ; Bernstein DI ; Bernstein JA ; Lummus ZL
• Description:
  Antigen specific histamine releasing factors (HRF), associated with diisocyanate (DI) induced asthma, were studied in 32 workers exposed to DI. All workers presented with occupational asthma (OA) associated with DI exposure. Venous blood was collected for characterization of peripheral blood mononuclear cells (PBMC), HRF, monocyte chemoattractant proteins (MCP), and the chemokine known as regulated on activation normal-T expressed and secreted (RANTES). Mean values for HRF in OA workers were greater than for asymptomatic exposed workers or unexposed controls after stimulation of PBMC with DI antigens. HRF was significantly associated with clinical diagnosis of OA. Specific immunoglobulin-E or immunoglobin-G antibody production did not correlate with OA. Hapten specific enhancement of HRF was significantly greater when PBMCs were stimulated in-vitro with the DI chemicals to which workers had been exposed. Immunoglobin-G antibodies, but not immunoglobulin-E, also demonstrated humoral immune response to DI antigens. Symptomatic workers showed higher mean production of antigen stimulated MCP and HRF than asymptomatic controls, but RANTES production was similar in both groups. A greater synthesis of antigen stimulated MCP messenger RNA occurred in an asthmatic worker than in a normal control. Purified T-cells from asymptomatic workers showed normal CD4/CD8 ratios (2.2), whereas low CD4/CD8 ratios (1.0 to 1.4) occurred in OA workers. The study confirmed that PBMC of workers with known OA induced by DI can be stimulated in-vitro to produce basophil activating HRF and MCP. The authors conclude that the HRF bioassay appears to detect a cell mediated immune response to DI haptens in workers with DI induced OA. [Description provided by NIOSH]
• Subjects:
  Asthma Asthma, Occupational Irritants Occupational Diseases Occupational Exposure Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Airway-resistance Bronchial-asthma Humans NIOSH-Grant NIOSH-Publication Occupational-diseases Occupational-exposure Occupational-hazards Occupational-health Pulmonary-system-disorders Respiratory-irritants

  More +
• ISSN:
  0300-483X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  191-206
• Volume:
  111
• Issue:
  1
• NIOSHTIC Number:
  nn:00234232
• Citation:
  Toxicology 1996 Jul; 111(1-3):191-206
• Contact Point Address:
  Internal Medicine University of Cincinnati Med C 231 Bethesda Ave. Cincinnati, Ohio 45267-0563
• Federal Fiscal Year:
  1996
• NORA Priority Area:
  Pulmonary System Disorders
• Performing Organization:
  University of Cincinnati, Cincinnati, Ohio
• Peer Reviewed:
  True
• Start Date:
  19880930
• Source Full Name:
  Toxicology
• End Date:
  19920331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:59006c1dcb604a5195e0542efd8071fc54f9560378f3bfe9c1e39a577bb8a94753bf08c5f018bf9e3aaf07b8b863a7623e86e81b78eedbfb3098b9d4659b3867
• Download URL:
  https://stacks.cdc.gov/view/cdc/197710/cdc_197710_DS1.pdf
• File Type:
  [PDF - 1.47 MB ]