Antioxidant Properties of Aspirin: Characterization of the Ability of Aspirin to Inhibit Silica-Induced Lipid Peroxidation, DNA Damage, NF-KB Activation, and TNF-Alpha Production

Details

• Personal Author:
  Castranova, Vincent ; Chen F ; Ding M ; Dong Z ; Leonard, Stephen S. ; Shi X ; Vallyathan V ; Wang S ; Ye J
• Description:
  Electron spin resonance (ESR) was used to investigate the reaction of aspirin toward reactive oxygen species, such as hydroxyl radicals (·OH), superoxide radicals ( O2 - ) and H2O2. The Fenton reaction (Fe(II) + H2O2 ---> FE(III) + -OH + OR) was used as a source of -OH radicals. The results show that aspirin is an efficient -OH radical scavenger with a reaction rate constant of k = 3.6 x 1010 M-1sec-1, which is faster than several well established antioxidants, such as ascorbate, glutathione and cysteine. However, aspirin is not a good scavenger for O2 - or H2O2. Through its antioxidant property, aspirin exhibited a protective effect against silica-induced lipid peroxidation and DNA strand breakage. Aspirin also inhibited the activation of nuclear transcription factor-b induced by silica, lipopolysaccharide or the transition metal, Fe(II), as demonstrated by electrophoretic mobility shift assay. The results show that aspirin functions as an antioxidant via its ability to scavenge -OH radicals. This antioxidant property may explain some of its various physiological and pharmacological actions. [Description provided by NIOSH]
• Subjects:
  Aluminum Silicates Antioxidants Carcinogens DNA Free Radicals Neoplasms Occupational Health Safety
• Keywords:
  Antioxidation Cancer Carcinogenesis Carcinogenicity DNA-damage Free-radicals Hydroxylation-reactions Medicinal-chemicals Peroxidases Silicates Statistical-analysis

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• ISSN:
  0300-8177
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Minnesota ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  93-102
• Volume:
  199
• Issue:
  1
• NIOSHTIC Number:
  nn:20027801
• Citation:
  Mol Cell Biochem 1999 Sep; 199(1):93-102
• CAS Registry Number:
  Aspirin (CAS RN 50-78-2) ; Quartz (SiO2) (CAS RN 14808-60-7)
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  True
• Source Full Name:
  Molecular and Cellular Biochemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6cf261906335122261bdc8a585fe84f188233102856edf079ff82fb0559355d9dbd48aeb440fe5866de03f5b042108f97b1f9a17e4d2778727c1a5d9255efc32
• Download URL:
  https://stacks.cdc.gov/view/cdc/197661/cdc_197661_DS1.pdf
• File Type:
  [PDF - 114.93 KB ]