Direct Detection of Histoplasma capsulatum in Soil Suspensions by Two-Stage PCR

Details

• Personal Author:
  Reid TM ; Schafer, Millie P.
• Description:
  Histoplasmosis is the most common pulmonary mycosis in the United States. The responsible fungal pathogen, Histoplasma capsulatum , grows in soils contaminated with bird or bat droppings. Inhalation of dust from contaminated areas containing H. capsulatum spores is a primary route of infection. The ability to detect H. capsulatum in soil samples has been limited by the lack of fast, reliable and inexpensive methods. A polymerase chain reaction (PCR) method was developed that allows the direct detection of H. capsulatum in soil. A two-stage PCR protocol was followed employing both fungal-specific primers and nested primers specific for the internal transcribed spacer (ITS) region of the 5·8S rRNA gene of H. capsulatum . The estimated limit of detection of this method is 10 spores. In contrast to the more expensive and indirect mouse inoculum assay, which requires 6-8 weeks for sample analysis, PCR analysis of soil contaminated with H. capsulatum can be completed in less than 2 days. [Description provided by NIOSH]
• Subjects:
  Dust Fungi Irritants Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Analytical-methods Dust-analysis Dust-exposure Dust-inhalation Dust-particles Dust-sampling Fungal-diseases Fungal-infections Laboratory-animals Pathogens Pulmonary-disorders Pulmonary-system Pulmonary-system-disorders Respiratory-irritants Respiratory-system-disorders Ribonucleic-acids Soil-sampling

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• ISSN:
  0890-8508
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  269-273
• Volume:
  13
• Issue:
  1
• NIOSHTIC Number:
  nn:20027693
• Citation:
  Mol Cell Probes 1999 Aug; 13(1):269-273
• Contact Point Address:
  Divisions of Biomedical and Behavioral Sciences, National Institute/Occupational Safety and Health, 4676 Columbia Parkway, Cincinnati, Ohio 45226
• Email:
  tar9@cdc.gov
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  True
• Source Full Name:
  Molecular and Cellular Probes
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0082b6e5bfb4b3ca413016c476f9a55518f5dbae331397d740176b3d94c89ca2b481fefad4fe2bb22ebc78d13ff2c483fe6cc075280fbde5a99e06529ec3f714
• Download URL:
  https://stacks.cdc.gov/view/cdc/197629/cdc_197629_DS1.pdf
• File Type:
  [PDF - 254.24 KB ]