Neoplastic Potential of Rat Tracheal Epithelial Cell Lines Induced by 1-Nitropyrene and Dibenzo(a, I)pyrene

Details

• Personal Author:
  Batelli L ; Ensell MX ; Hubbs A ; Nath J ; Ong T ; Zhou G
• Description:
  Our previous study showed that both 1-nitropyrene (1-NP) and dibenzo(a,i)pyrene (DBP) induced enhanced growth variants (EGVs) in primary cultures of rat tracheal epithelial (RTE) cells exposed in vivo. Cell lines were established from some of the EGVs. Further studies, using anchorage-independent growth in soft agar and tumorigenicity in athymic nude mice, were performed to determine the neoplastic potential of EGVs induced by 1-NP and DBP. Results show that three of five from DBP- and five of five from 1-NP-induced cell lines displayed anchorage-independent growth. The colony forming efficiency (CFE) from DBP-induced cell lines was 0.067 per thousand and CFE from 1-NP-induced cell lines was 0.151 per thousand. There is a significant difference between the two CFEs (mu = 12.08, P<0. 01). Two of five DBP- and five of five 1-NP-induced cell lines produced squamous cell carcinomas (SCC) in nude mice. The rate of tumorigenicity counted by injected sites was 20% (6/30) for DBP-induced cell lines and 57% (17/30) for 1-NP-induced cell lines. There is a significant difference between the results of tumorigenicity from the cell lines induced by the two different compounds (chi(2)=8.53, P<0.01). Neither of the two cell lines from spontaneously developed foci grew in soft agar or produced SCC in nude mice. It seems that the neoplastic potential of transformed RTE cells induced by 1-NP was higher than that of DBP. [Description provided by NIOSH]
• Subjects:
  Animals Benzopyrenes Carcinogens Cell Division Hazardous Substances Nervous System Occupational Health Safety
• Keywords:
  Analytical-methods Analytical-models Analytical-processes Animal-studies Carcinogenicity Cell-division Cell-transformation Neoplastic-agents Statistical-analysis Toxic-materials
• ISSN:
  0027-5107
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  193-199
• Volume:
  444
• Issue:
  1
• NIOSHTIC Number:
  nn:20027683
• Citation:
  Mutat Res 1999 Jul; 444(1):193-199
• Contact Point Address:
  Toxicology and Molecular Biology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, MS 3014, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  too2@cdc.gov
• CAS Registry Number:
  1-Nitropyrene (CAS RN 5522-43-0) ; Dibenzo[a,i]pyrene (CAS RN 189-55-9)
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  True
• Source Full Name:
  Mutation Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e6888ba7cb64bc0c113cb670eb29b0b074ba9b05228c73e047c12d0b760d9be5d55c9839968fb5bc97d7ebb33ba59d41f046e6becf747d564cbe0014b202af53
• Download URL:
  https://stacks.cdc.gov/view/cdc/197624/cdc_197624_DS1.pdf
• File Type:
  [PDF - 80.63 KB ]