A Quantitative In Vitro Fluorescence Imaging Method for Phospholipid Loss from Respirable Mineral Particles

Details

• Personal Author:
  Keane MJ ; Wallace WE
• Description:
  Respirable quartz and kaolin particles were treated with fluorescent-labeled phospholipids to model contact of fibrogenic and nonfibrogenic particles with pulmonary surfactant in the alveolar regions of the lung. Particles were used to challenge rat pulmonary macrophages in vitro at times from 1 d to 10 d. The objective was to develop a quantitative method to track surfactant components that adsorb to respirable particles in the lung or inside cells. Confocal laser scanning microscopy was used to image and quantify surfactant remaining on particles internalized by cells. Results indicate that the fluorescent label is removed from quartz particles quickly, with the fluorescence intensity less than 15% of initial value at 3 d, and about 5% at 10 d. In contrast, the kaolin particle-associated fluorescence was still approximately 39% of initial intensity at 3 d, and 10-15% at 10 d. Unchallenged cells showed a background of approximately 5%, and noninternalized particles did not exhibit any loss of fluorescence over the 10-d exposure. The results indicate the method may be useful in label-removal rate studies of respirable particles in vitro, with some cautions and limitations. Results are discussed and compared with similar studies using nonimaging techniques. [Description provided by NIOSH]
• Subjects:
  Animals Dust Environmental Monitoring Laboratories Lipids Lung Diseases Minerals Occupational Health Quartz Respiratory Tract Diseases Safety Silicon Dioxide Surface-Active Agents

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• Keywords:
  Animal-studies Exposure-assessment Exposure-levels In-vitro-studies Laboratory-animals Lung-disorders Phospholipids Pulmonary-system-disorders Quantitative-analysis Quartz-dust Respirable-dust Respiratory-system-disorders Surfactants

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• ISSN:
  0895-8378
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  287-292
• Volume:
  17
• Issue:
  6
• NIOSHTIC Number:
  nn:20026633
• Citation:
  Inhal Toxicol 2005 May; 17(6):287-292
• CAS Registry Number:
  Kaolin (CAS RN 1332-58-7) ; Quartz (SiO2) (CAS RN 14808-60-7)
• Federal Fiscal Year:
  2005
• NORA Priority Area:
  Work Environment and Workforce: Mixed Exposures
• Peer Reviewed:
  True
• Source Full Name:
  Inhalation Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:01e150bd59b76dcb104d34b8354251207bc1ccb5ffd9674d800a6854c8ebd8f52dd967fe4a667061908b689025853e1d30b0334343beaae06ae758ada9c78c8a
• Download URL:
  https://stacks.cdc.gov/view/cdc/197343/cdc_197343_DS1.pdf
• File Type:
  [PDF - 692.66 KB ]