Evaluation of Malignant Pleural Mesothelioma Using Morphometry and Immunohistochemistry

Details

• Personal Author:
  Antao VC ; Capelozzi VL ; Monteiro MM ; Pinheiro GA ; Saldiva P ; Terra-Filho M
• Description:
  Objective: To analyze the significance of morphometry and immunohistochemistry in the prognosis of malignant pleural mesothelioma. Methods: Histopathologic material was obtained from 51 decedents who had been diagnosed with pleural tumors in the state of Rio de Janeiro between 1979 and 2000. Pleural tumor biopsies had been routinely fixed in formaldehyde and embedded in paraffin. In 34 of these cases, there was enough material for immunohistochemistry analysis following a slightly modified ABC-method to assay for calretinin, thrombomodulin, CEA, CD15, p53 and Ki-67. The stereological method of "point-sampled intercepts" was used. Results: The most common histopathologic pattern of the mesotheliomas diagnosed was epithelioid (27 cases, 60%). The median survival period for mesotheliomas was 6 months and the 5-year survival rate was 3%. Using Cox proportional hazards model, only 3 variables provided additional prognostic information: female sex (median survival=4 months; p=0.04) and percents of tumor cells staining for thrombomodulin and for p53. Thrombomodulin staining of more than 3.9% mesothelioma cells, and p53 staining of 12% or more, correlated with poor survival (median=3 and 4 months, respectively, p=0.05). For Ki-67, nuclear staining of more than 17.7% of the cells indicated low survival, but the difference was not statistically significant. Conclusions: The identification of thrombomodulin expression and biologic features related to nuclear activity in tumor tissue from patients diagnosed with pleural mesothelioma may have clinical relevance. [Description provided by NIOSH]
• Subjects:
  Epidemiology Lung Lung Diseases Mortality Neoplasms Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Lung-cancer Lung-disease Lung-disorders Mortality-data Mortality-rates Mortality-surveys Pulmonary-system-disorders Respiratory-system-disorders Statistical-analysis Surveillance
• ISSN:
  1073-449X
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  169
• Issue:
  7
• NIOSHTIC Number:
  nn:20026036
• Citation:
  Am J Respir Crit Care Med 2004 Apr; 169(7)(Abstracts):A645
• Email:
  ghp6@CDC.GOV
• Federal Fiscal Year:
  2004
• Peer Reviewed:
  False
• Source Full Name:
  American Journal of Respiratory and Critical Care Medicine
• Supplement:
  Abstracts
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:fd89df90d979d3de4e16bc316a6910c7025f31457f925225c06b2bd4fe347450fdf200f109c72678deec6a29f013a5b9a31eb433833211a1b5014c9c4f058c7b
• Download URL:
  https://stacks.cdc.gov/view/cdc/197161/cdc_197161_DS1.pdf
• File Type:
  [PDF - 388.80 KB ]