Airway Hyperreactivity Elicited by Toluene Diisocyanate (TDI)-Albumin Conjugate Is Not Accompanied by Airway Eosinophilic Infiltration in Guinea Pigs

Details

• Personal Author:
  Fedan, Jeffery S. ; Frazer DG ; Huang J ; Millecchia LL
• Description:
  Nonspecific airway hyperresponsiveness is present in many patients with toluene diisocyanate (TDI)-induced asthma; however, the underlying pathophysiological mechanisms of this hyperresponsiveness remain controversial. In the present study, we used a guinea pig model to investigate the association of TDI-induced airway hyperresponsiveness with eosinophilic airway infiltration, which is widely considered to play a key role in the development of allergen-induced hyperresponsiveness. Guinea pigs were sensitized by i.d. injections of 10 microl TDI on day 1 and day 6. Control animals received saline injections. Two weeks after the second injection, airway reactivity to inhaled methacholine and specific airway resistance (sRaw) was measured before and at several times after inhalation challenge with TDI-GSA (guinea pig serum albumin) conjugates. Eosinophils in the airways were detected using enzyme histochemistry and quantified using computer-assisted image analysis. TDI-specific IgG1 antibodies were found in the blood of TDI-sensitized animals. An immediate increase in sRaw was induced in these animals by TDI-GSA challenge; airway hyperresponsiveness to methacholine was observed at 6 h and 18 h after TDI-GSA challenge. However, TDI-GSA challenge did not result in an elevation of eosinophils in the airways, compared with control animals. The results suggest that the development of TDI-induced airway hyperresponsiveness is not dependent upon eosinophil infiltration in airways. [Description provided by NIOSH]
• Subjects:
  Animals Asthma Asthma, Occupational Hypersensitivity Irritants Laboratories Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Airway-obstruction Airway-resistance Airway Hyperreactivity Airway Obstruction Allergens Animal-studies Author Keywords: Toluene Diisocyanate Eosinophil Guinea Pig Inhalation-studies Laboratory-animals Respiratory-irritants Respiratory-system-disorders

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• ISSN:
  0340-5761
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  141-146
• Volume:
  72
• Issue:
  3
• NIOSHTIC Number:
  nn:20026011
• Citation:
  Arch Toxicol 1998 Feb; 72(3):141-146
• Contact Point Address:
  Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Rd., Morgantown, WV 26505-2845, USA
• CAS Registry Number:
  Toluene 2,4-diisocyanate (CAS RN 584-84-9)
• Federal Fiscal Year:
  1998
• Peer Reviewed:
  True
• Source Full Name:
  Archives of Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:56845a4169db763b17c2ecd952a9cc13bcd0df68120ad73b046731119635b6c5bdc3cc566f820e84cf6e0f5d15ceb5ee2241a13cec414cda9202acfe5cf1884c
• Download URL:
  https://stacks.cdc.gov/view/cdc/197145/cdc_197145_DS1.pdf
• File Type:
  [PDF - 304.81 KB ]