Chemokine Receptor CCR2 Involvement in Skeletal Muscle Regeneration

Details

• Personal Author:
  Gao X ; Hulderman T ; Kuziel WA ; Millecchia L ; Mishra D ; O'Farrell L ; Simeonova PP ; Warren GL
• Description:
  Chemokines, signaling through the CCR2 receptor, are highly expressed in injured skeletal muscle. Their target specificity depends on the cellular expression of the specific receptors. Here we demonstrate that, in freeze-injured muscle, CCR2 co-localized with Mac-3, a marker of activated macrophages as well as with myogenin, a marker of activated muscle precursor cells. The degeneration/regeneration process in skeletal muscle of CCR2-/- and wild-type mice was not significantly different at day 3. However in contrast to the regenerated muscle of the wild-type mice, the muscle from CCR2-/- mice was characterized by impaired regeneration, inflammation, and fibrotic response at day 14, increased fat infiltration, fibrosis, and calcification at day 21, and impaired strength recovery until at least 28 days post-injury. Consistently, the increased expression of Mac-1 and TNF-a was prolonged in the injured muscle of CCR2-/- mice. The expression pattern of the myogenic factors MyoD and myogenin was similar for both types of mice, while NCAM, which is associated with the initiation of fusion of muscle precursor cells, was more increased in the injured muscle of CCR2-/- mice. In conclusion, the study delineates that signaling through CCR2 is involved in muscle precursor cell activities necessary for complete and rapid regeneration of injured skeletal muscle. [Description provided by NIOSH]
• Subjects:
  Animals Fibrosis Laboratories Musculoskeletal System Occupational Health Safety
• Keywords:
  Adipogenesis Animal-studies Author Keywords: Skeletal Muscle Injury CCL2 Inflammation Laboratory-animals Muscle-cells Muscles Myogenesis Skeletal-system
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  California ; Georgia ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  413-415
• Volume:
  19
• Issue:
  3
• NIOSHTIC Number:
  nn:20025892
• Citation:
  FASEB J 2005 Mar; 19(3):413-415
• Email:
  PSimeonova@cdc.gov
• Federal Fiscal Year:
  2005
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  True
• Source Full Name:
  The FASEB Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3b5fc753bf1d24c23eb94ef379554e47bd41709744c0ab59b619a0abfee475e0f83827c4c4532fa7d7a0583bfd67dd9815328916a84a99d1053f3c9b848ef322
• Download URL:
  https://stacks.cdc.gov/view/cdc/197079/cdc_197079_DS1.pdf
• File Type:
  [PDF - 7.42 MB ]