Contact Hypersensitivity to Dicyclohexylcarbodiimide and Diisopropylcarbodiimide in Female B6C3F1 Mice

Details

• Personal Author:
  Gerber PC ; Griffey SS ; Hayes BB ; Meade BJ
• Description:
  Dicyclohexylcarbodiimide (DCC) and diisopropylcarbodiimide (DIC) are two commonly used coupling reagents in protein synthesis resulting in exposure of individuals in chemical and pharmaceutical industries as well as research laboratories involved in protein synthesis and recombinant DNA techniques. The objectives of these studies were to determine the irritation and sensitizing potential of these two compounds when applied topically to B6C3F1 mice. Sensitization potential was assessed by the Mouse Ear Swelling Test (MEST) and the murine Local Lymph Node Assay (LLNA). Concentrations used in the contact hypersensitivity assays were determined by primary irritancy studies. DCC and DIC were identified as both irritants and contact sensitizers with the MEST being a more sensitive indicator of sensitization potential. The MEST identified DCC as a sensitizer at concentrations as low as 0.006% (w/v) 24 hr and 48 hr post challenge and DIC at 0.3% (w/v) and 1.5% (w/v) 24 and 48 hr post challenge, respectively. In the LLNA, the lowest concentrations yielding a significant response were 0.06% (w/v) for DCC and 10% (w/v) for DIC. [Description provided by NIOSH]
• Subjects:
  Animals DNA Irritants Laboratories Occupational Health Pharmacies Safety Skin
• Keywords:
  Animal-studies Deoxyribonucleic-acids Laboratory-animals Pharmaceutical-industry Protein-chemistry Protein-synthesis Proteins Skin-exposure Skin-irritants
• ISSN:
  0148-0545
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  195-206
• Volume:
  21
• Issue:
  2
• NIOSHTIC Number:
  nn:20025314
• Citation:
  Drug Chem Toxicol 1998 May; 21(2):195-206
• Federal Fiscal Year:
  1998
• Peer Reviewed:
  True
• Source Full Name:
  Drug and Chemical Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5214891181a4a30a6e56485c5b7835a341fa2f45932ff54c2e8e5336f42fec254a162c25fb8db8f0927879f90ab5a2a3be33f57205c8fcf8161f91c7590add76
• Download URL:
  https://stacks.cdc.gov/view/cdc/196750/cdc_196750_DS1.pdf
• File Type:
  [PDF - 743.91 KB ]