Altered Alveolar Macrophage Function in Calorie-Restricted Rats

Details

• Personal Author:
  Dong W ; Gilmour MI ; Kari FW ; Lange RW ; Luster MI ; Park P ; Selgrade MK
• Description:
  Alveolar macrophage functions associated with clearance of bacteria from the lung were assessed in male Fischer 344 rats maintained on a 25% calorie-restricted diet. Calorie-restricted and ad libitum-fed (control) rats were exposed to concentrations of ozone known to compromise phagocytic function of alveolar macrophages. Ozone suppressed alveolar macrophage phagocytosis of latex beads in vitro in ad libitum-fed rats, but not in calorie-restricted rats. In fact, caloric restriction enhanced phagocytic function in both control and ozone-exposed animals. Ad libitum-fed rats exposed to ozone and challenged with Streptococcus zooepidemicus experienced a prolonged infection and influx of polymorphonuclear leukocytes (PMN), whereas calorie-restricted rats exposed to ozone cleared the bacteria in 24 h without an inflammatory response. Bacterial endotoxin-stimulated in vitro production of nitric oxide and tumor necrosis factor (TNF)-alpha as well as expression of TNF-alpha and interleukin-6 messenger RNAs were all lower in alveolar macrophages isolated from calorie-restricted rats. Together, the data suggest that caloric restriction enhances resistance to gram-positive bacteria, while lowering the production of proinflammatory mediators elicited by endotoxin, a component of gram-negative bacteria. Although increased bacterial resistance is considered beneficial, reduction in the lung's ability to induce inflammatory mediators can have both positive and pathophysiologic consequences. [Description provided by NIOSH]
• Subjects:
  Animals Laboratories Lung Lung Diseases Microbiology Occupational Health Respiratory System Safety
• Keywords:
  Alveolar-cells Animal-studies Bacteria Exposure-levels In-vitro-studies Laboratory-animals Lung-disorders Microorganisms Phagocytes Phagocytic-activity
• ISSN:
  1044-1549
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  462-469
• Volume:
  19
• Issue:
  3
• NIOSHTIC Number:
  nn:20025294
• Citation:
  Am J Respir Cell Mol Biol 1998 Sep; 19(3):462-469
• Contact Point Address:
  Frank W. Kari, Ph.D., NIEHS, M.D. B3-09, P.O. Box 12233, Research Triangle Park, NC 27709
• Email:
  kari@niehs.nih.gov
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9)
• Federal Fiscal Year:
  1998
• Peer Reviewed:
  True
• Source Full Name:
  American Journal of Respiratory Cell and Molecular Biology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:811852bd8440cbe5395b2131890dd139f7c40e8ca3b946428644de59a86f6d44ec3d4bcfd60b48fe4b3c155d5f95749235cdd451957efd93404841dc7567e144
• Download URL:
  https://stacks.cdc.gov/view/cdc/196732/cdc_196732_DS1.pdf
• File Type:
  [PDF - 200.49 KB ]