Concentration- and Time-Dependent Upregulation and Release of the Cytokines MIP-2, KC, TNF, and MIP-1alpha in Rat Alveolar Macrophages by Fungal Spores Implicated in Airway Inflammation

Details

• Personal Author:
  Lewis, Daniel M. ; Morey R ; Paulauskis JD ; Shahan TA ; Sorenson WG
• Description:
  Inhalation of fungal spores has been shown to cause primary or secondary infection and respiratory inflammation and diseases such as allergic alveolitis, atopic asthma, and organic dust toxic syndrome, which are rarely reported in the absence of predisposing factors. Biochemical and molecular markers of inflammation were measured in rat bronchial alveolar lavage cells (> 95% macrophages) following stimulation with fungal spores isolated from pathogenic and nonpathogenic fungi that have been implicated in airway inflammation. The results of this study demonstrate that mRNA transcripts for the C-X-C branch of the PF4 superfamily are differentially upregulated over those of the C-C mediators in a time- and concentration-dependent manner. Macrophage inflammatory protein (MIP)-2 and KC were differentially upregulated over the acute phase inflammatory cytokines MIP-1alpha and tumor necrosis factor-alpha (TNF-alpha) in rat alveolar macrophages stimulated with fungal spores from Aspergillus candidus, Aspergillus niger, Eurotium amstelodami, and Cladosporium cladosporioides. Spores from Aspergillus terreus and Penicillium spinulosum failed to stimulate an increase of any cytokine mRNA, whereas those from Aspergillus fumigatus stimulated the upregulation of MIP-2, KC, TNF-alpha, and MIP-1alpha mRNAs. Over time, A. fumigatus stimulated increasing KC production until 24 h, when production levels increased slightly, then leveled off when measurements ceased at 36 h. Latex spheres stimulated modest amounts of MIP-2 and transforming growth factor-beta only. These observations suggest that the inflammatory cytokines MIP-2 and KC may be involved in the inflammation arising from the inhalation of fungal spores in a time- and concentration-dependent manner. [Description provided by NIOSH]
• Subjects:
  Aerosols Animals Asthma Asthma, Occupational Dust Fungi Hypersensitivity Laboratories Lung Microbiology Occupational Health Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Airway-obstruction Allergic-disorders Alveolar-cells Animal-studies Bronchial-asthma Fungal-infections Laboratory-animals Microorganisms Organic-dusts Respiratory-system-disorders
• ISSN:
  1044-1549
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Massachusetts ; OSHA Region 1 ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  435-440
• Volume:
  18
• Issue:
  3
• NIOSHTIC Number:
  nn:20025293
• Citation:
  Am J Respir Cell Mol Biol 1998 Mar; 18(3):435-440
• Contact Point Address:
  W.G. Sorenson, 1095 Willowdale Road, MS-215, CDC/DRDS/NIOSH, Morgantown, WV 26505
• Email:
  wgs1@cdc.gov
• Federal Fiscal Year:
  1998
• Peer Reviewed:
  True
• Source Full Name:
  American Journal of Respiratory Cell and Molecular Biology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:18039fe46a0fdaf3d70e6dba29c73cc149b986e4584ceb441c2d10cb93fcfd18e43dd79b4c816e218d981eee7cb57071eeeda9305aba1ed0c65aa19bf1613007
• Download URL:
  https://stacks.cdc.gov/view/cdc/196731/cdc_196731_DS1.pdf
• File Type:
  [PDF - 140.07 KB ]