Immunohistochemical Localization and Quantification of Glial Fibrillary Acidic Protein and Synaptosomal-Associated Protein (Mol. Wt 25000) in the Ageing Hippocampus Following Administration of 5,7-Dihydroxytryptamine

Details

• Personal Author:
  Dugar A ; Lakoski JM ; O'Callaghan JP ; Patanow C
• Description:
  Responses to injury in the ageing hippocampus were assessed utilizing the synaptic markers glial fibrillary acidic protein and synaptosomal-associated protein (mol. wt 25,000) following administration of the neurotoxin, 5,7-dihydroxytryptamine, into the fimbria-fornix and cingulum bundle to denervate serotonergic afferent input to the dorsal hippocampus. Age-dependent alterations in hippocampal immunohistochemical localization of glial fibrillary acidic protein and synaptosomal-associated protein were evaluated in female Fischer 344 rats following serotonergic deafferentation with 5,7-dihydroxytryptamine. Across the lifespan, as indicated by measurements taken at three, 18, 21 and 29 months, marked increases in glial fibrillary acidic protein, but not synaptosomal-associated protein immunoreactivity, occurred throughout the hippocampus at 21 and 29 months compared to three and 18 months. Following three weeks pretreatment with 5,7-dihydroxytryptamine (20 microg total dose) or vehicle (0.1% ascorbic saline; 2 microl total volume) infused in the fimbria-fornix/cingulum bundle, immunohistochemical analysis demonstrated marked increases of glial fibrillary acidic protein, but not synaptosomal-associated protein, in the 18-month 5,7-dihydroxytryptamine group compared to the 18-month vehicle and 3-month 5,7-dihydroxytryptamine groups. Additionally, a significant increase in glial fibrillary acidic protein concentration was found by enzyme-linked immunosorbent assay in the 18-month 5,7-dihydroxytryptamine group compared to the 18-month vehicle and three-month 5,7-dihydroxytryptamine groups. These results demonstrate that selective neurotoxicant damage of the hippocampal serotonergic system differentially alters the expression of glial fibrillary acidic protein. This approach may provide a valuable tool to determine the ability of the hippocampus to respond to age-related neurodegenerative injury. [Description provided by NIOSH]
• Subjects:
  Age Factors Aging Animals Immune System Nervous System Occupational Health Safety
• Keywords:
  5,7-dihydroxytryptamine Age-factors Age-groups Animal-studies Author Keywords: Serotonin (5-hydroxytryptamine) Brain-function Brain-matter Fimbria-fornix/Cingulum Bundle Glial Fibrillary Acidic Protein Hippocampus Immune-reaction Immune-system Neuropathy Neurotoxic-effects Neurotoxicity Neurotoxins Neurotransmitters Proteins Synaptosomal-associated Protein (mol. Wt 25,000)

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• ISSN:
  0306-4522
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  123-133
• Volume:
  85
• Issue:
  1
• NIOSHTIC Number:
  nn:20025237
• Citation:
  Neuroscience 1998 Jul; 85(1):123-133
• Contact Point Address:
  J.M. Lakoski Centers for Disease Control and Prevention, NIOSH, Health Effects Laboratory Division TMBB (MS 3014), 1095 Willowdale Road, Morgantown, WV 26505-2888, USA
• Federal Fiscal Year:
  1998
• Peer Reviewed:
  True
• Source Full Name:
  Neuroscience
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d67cb554461f7669e13490a77c5de891bf6f9f80ad6df2155f56edd28daea6fde2463c08fe2a499dea149913b11adb9a48aafecff012ec13ca6765d15cebc59f
• Download URL:
  https://stacks.cdc.gov/view/cdc/196700/cdc_196700_DS1.pdf
• File Type:
  [PDF - 305.67 KB ]