Inhibition of Nuclear Factor Kappa B by Phenolic Antioxidants: Interplay Between Antioxidant Signaling and Inflammatory Cytokine Expression

Details

• Personal Author:
  Kinneer K ; Ma Q
• Description:
  Phenolic antioxidants inhibit the induction of inflammatory cytokines by inflammatory stimuli. Here, we analyzed the mechanism by which the antioxidants inhibit LPS-induced expression of TN approximately in macrophages. Hydroquinone (HQ) and t-butyl hydroquinone (tBHQ), prototypes of phenolic antioxidants, block LPS-induced transcription of TN approximately and a NF-kB-mediated reporter gene expression, suggesting NF-kB as a target in the inhibition. Analyses of the NF- kB activation pathway revealed that the antioxidants do not inhibit LPS-induced activation of the IkB kinase activity, degradation of IkB approximately or translocation of activated NF-kB into the nucleus, but block the formation of NF-k B/DNA binding complexes. In vitro experiments showed that the antioxidants do not directly interfere with DNA binding of NF-kB. Structure-activity analyses suggest that inhibition of NF-kB function involves the redox cycling property of the antioxidants. These findings implicate a redox-sensitive factor important for the binding of NF-kB to its DNA recognition sequence as a target molecule in the inhibition of NF-kB function and inflammatory cytokine expression by phenolic antioxidants. [Description provided by NIOSH]
• Subjects:
  Antioxidants Genetics Occupational Health Phenol Safety
• Keywords:
  Cell-damage Cellular-reactions Cellular-structures Genes In-vitro-studies Phenols Subcellular-structure
• ISSN:
  1096-6080
• Publisher:
  Society of Toxicology
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Maryland ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  78
• NIOSHTIC Number:
  nn:20025158
• Citation:
  Toxicologist 2004 Mar; 78(S-1):324
• CAS Registry Number:
  Hydroquinone (CAS RN 123-31-9)
• Federal Fiscal Year:
  2004
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 43nd Annual Meeting and ToxExpo, March 21-25, 2004, Baltimore, Maryland
• Supplement:
  S-1
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f5e939511293b85799f1ca06e1157ad4c042f0da9b50f749553fba99cf3943d4dcfb9881956193ef15051ed38fac1e60af466f6a2965727d4bf9b964d62ca211
• Download URL:
  https://stacks.cdc.gov/view/cdc/196644/cdc_196644_DS1.pdf
• File Type:
  [PDF - 56.60 KB ]