Mutagenicity of 4.4’methylene-Bis-2-Chloroanaline (MOCA) and 2-Phenyl-1.4-Benzoquinone (PBQ) in Human Lympho-Blastoid Cells

Details

• Personal Author:
  Cheever KL ; DeBord, D. Gayle ; Reid TM ; Savage RE Jr.
• Description:
  The genotoxic potential of two occupationally significant chemicals, 4.4'methylene-bis-2-chloroanaline (MOCA) and 2-phenyl-1.4-benzoquinone (PBQ), was explored by monitoring the induction of mutations at the HPRT locus of AHH-l human lymphoblastoid cells. In order to bind to DNA, MOCA requires activation via N-oxidation to N-hydroxy-MOCA (N-OH- MOCA). Exposure of AHH-l cells to N-OH-MOCA induced a 6-fold increase in mutant frequency and resulted in base pair substitutions primarily at A:T base pairs. In contrast, exposure to PBQ did not result in an increased mutant frequency although this compound was significantly more cytotoxic than N- OH-MOCA at equimolar doses. The induction of mutations at A:T sites by N-OH-MOCA is consistent with the type of DNA damage known to be produced by MOCA and provides a specific marker of genotoxic damage for exposed populations. [Description provided by NIOSH]
• Subjects:
  DNA Occupational Health Safety
• Keywords:
  Cell-transformation Cellular-function Cellular-structures DNA-damage Genotoxic-effects Mutation
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Ohio ; OSHA Region 10 ; OSHA Region 5 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  80
• Volume:
  42
• NIOSHTIC Number:
  nn:20025125
• Citation:
  Toxicologist 1998 Mar; 42(1-S):80
• Federal Fiscal Year:
  1998
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 37th Annual Meeting, March 1-5,1998, Seattle, Washington
• Supplement:
  1-S
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:66da2f420bc11238ed0d727b6f864f0c0eb60d2a27efcd23fda85d4ef782557b55cc843143858a0880a770b4fc1bcdaf3475038859a8d85425f08f0d3ef5e0a5
• Download URL:
  https://stacks.cdc.gov/view/cdc/196615/cdc_196615_DS1.pdf
• File Type:
  [PDF - 141.69 KB ]