Electric Field-Induced Changes in Agonist-Stimulated Calcium Fluxes of Human HL-60 Leukemia Cells

Details

• Personal Author:
  Cleary SF ; Conover, David L. ; Kim YV ; Lotz, W. Gregory
• Description:
  The mechanism of biological effects of extremely-low-frequency electric and magnetic fields may involve induced changes of Ca2+ transport through plasma membrane ion channels. In this study we investigated the effects of externally applied, low-intensity 60 Hz electric (E) fields (0.5 V/m, current density 0.8 A/m2) on the agonist-induced Ca2+ fluxes of HL-60 leukemia cells. The suspensions of HL-60 cells received E-field or sham exposure for 60 min and were simultaneously stimulated either by 1 microM ATP or by 100 microM histamine or were not stimulated at all. After E-field or sham exposure, the responses of the intracellular calcium levels of the cells to different concentrations of ATP (0.2-100 microM) were assessed. Compared with control cells, exposure of ATP-activated cells to an E-field resulted in a 20-30% decrease in the magnitude of [Ca2+]i elevation induced by a low concentration of ATP (<1 microM). In contrast, exposure of histamine-activated HL-60 cells resulted in a 20-40% increase of ATP-induced elevation of [Ca2+]i. E-field exposure had no effect on non-activated cells. Kinetic analysis of concentration-response plots also showed that compared with control cells, exposure to the E-field resulted in increases of the Michaelis constant, Km, value in ATP-treated cells and of the maximal [Ca2+]i peak rise in histamine-treated HL-60 cells. The observed effects were reversible, indicating the absence of permanent structural damages induced by acute 60 min exposure to electric fields. These results demonstrate that low-intensity electric fields can alter calcium distribution in cells, most probably due to the effect on receptor-operated Ca2+ and/or ion channels. [Description provided by NIOSH]
• Subjects:
  Calcium Compounds Cells, Cultured Magnetic Fields Occupational Health Safety
• Keywords:
  Author Keywords: 60 Hz Electric Field Bioelectric-effects Biological-effects Calcium-compounds Cell-cultures Exposure-levels Histamine Receptor Intracellular Free Calcium Concentration Magnetic-fields Purinergic Receptor Receptor-operated Ion Channels Simulation-methods

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• ISSN:
  0197-8462
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 3 ; OSHA Region 5 ; Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  366-376
• Volume:
  19
• Issue:
  6
• NIOSHTIC Number:
  nn:20024977
• Citation:
  Bioelectromagnetics 1998 Aug; 19(6):366-376
• Contact Point Address:
  David L. Conover, C-27, Division of Biomedical and Behavioral Science, National Institute for Occupational Safety and Health, 4676 Columbia Parkway, Cincinnati, OH 45226
• Federal Fiscal Year:
  1998
• Peer Reviewed:
  True
• Source Full Name:
  Bioelectromagnetics
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:16e88ac519751457060bdc339e28c12dc4dc4feb43343acdcdf41a6ce2179912f70db72eb7e6f1d6887f6ecfae8ea9a93438396ffb35f5c1e0b763120899269e
• Download URL:
  https://stacks.cdc.gov/view/cdc/196518/cdc_196518_DS1.pdf
• File Type:
  [PDF - 245.18 KB ]