Molecular Characterization of a Voltage-Gated Potassium Channel Expressed in Rat Testis

Details

• Personal Author:
  Benoff S ; Cooper GW ; Goodwin LO ; Hurley IR ; Jacob A
• Description:
  Potassium (K(+)) channels are present in both mammalian testis and spermatozoa. Immunofluorescent detection of sperm-bound biotinylated charybdotoxin, an inhibitor of Ca(2+)-activated and of delayed rectifier K(+) channels, indicated that these ion channels are uniformly distributed over the surface of both heads and tails of unfixed rat epididymal spermatozoa. Reverse transcription-polymerase chain reaction (RT-PCR) analysis on rat testis RNA with PCR primers, based on known nucleotide sequences of different classes of K(+) channels, amplified sequences homologous to delayed rectifier K(+) channels. In-situ RT-PCR on rat testis sections showed that these K(+) channel transcripts are present in the cytoplasm of primary spermatocytes and post-meiotic elongating spermatids. Northern blot analysis of various rat tissues identified multiple K(+) channel transcripts, some of which were observed only in testis. An attempt to obtain a full length rat testis K(+) channel cDNA sequence gave an assembled sequence of 2693 base pairs with >90% homology to a delayed rectifier K(+) channel, Kv1.3. A method for rapid amplification of cDNA ends was employed to amplify the 5' sequences of the rat testis cDNA but a unique sequence could not be obtained. DNA sequencer traces suggest that multiple related K(+) channels which differed at their 5' ends were amplified in rat testis. [Description provided by NIOSH]
• Subjects:
  Animals Biomarkers Laboratories Occupational Health Potassium Compounds Safety Spermatogenesis Urogenital Diseases Urogenital System
• Keywords:
  Animal-studies Author Keywords: Charybdotoxin Labelling Fertility Laboratory-animals Morphology Potassium-compounds Potassium Channel Reproductive-hazards Reproductive-system Spermatozoa Sperm Membrane Testis

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• ISSN:
  1360-9947
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  303-313
• Volume:
  6
• Issue:
  4
• NIOSHTIC Number:
  nn:20024662
• Citation:
  Mol Hum Reprod 2000 Apr; 6(4):303-313
• Contact Point Address:
  North Shore University Hospital, 300 Community Drive, Boas-Marks Biomedical Science Research Center - Room 119, Manhasset, New York 11030, USA
• CAS Registry Number:
  Potassium (CAS RN 7440-09-7)
• Federal Fiscal Year:
  2000
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Performing Organization:
  North Shore University Hospital, Manhasset, New York
• Peer Reviewed:
  False
• Start Date:
  19980701
• Source Full Name:
  Molecular Human Reproduction
• End Date:
  20000630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1c4aa3da98fb25ddf0e28dc46c559c8c0cd14096a5acc8930c082dd6a727a0868c7abf517a64cb7b25f01826c9fd41c871213acf03cbd04068540251eea10ff1
• Download URL:
  https://stacks.cdc.gov/view/cdc/196348/cdc_196348_DS1.pdf
• File Type:
  [PDF - 664.28 KB ]