Double Probing of Human Spermatozoa for Persistent Histones, Surplus Cytoplasm, Apoptosis and DNA Fragmentation

Details

• Personal Author:
  Bennett D ; Demir R ; Huszar G ; Ovari L ; Sati L ; Simon SD
• Description:
  Individual spermatozoa were assessed with pairs of probes for persistent histones and cytoplasmic retention, persistent histones and DNA fragmentation, and persistent histones and apoptotic markers. The individual spermatozoa were treated sequentially with combinations of probes for these cytoplasmic and nuclear biochemical markers. Sperm fields were recorded with computer-assisted imaging, and staining patterns with the two probes in the same spermatozoa were examined and scored as light, intermediate or dark (mature to arrested-maturity spermatozoa). The effects of arrested sperm maturation were similar with respect to the cytoplasmic and nuclear characteristics of spermatozoa in 84% of cells, indicating that cytoplasmic and nuclear attributes of arrested sperm maturation are related. However, there were moderate (intermediate-dark or intermediate-light patterns, 14.5% of cells) or major (light-dark patterns, 1.6% of cells) discrepancies in the intensity of the double staining patterns. Thus, testing with single maturity markers may not be fully reliable. These findings are important with respect to: (i) arrested sperm maturation; (ii) potential efficacy of antioxidant and similar therapeutic strategies in subfertile men, as spermatozoa with infrastructure defects due to mismaturation or maturation arrest are unlikely to respond to interventions; and (iii) detection of adverse male environmental exposures. [Description provided by NIOSH]
• Subjects:
  Biomarkers Environmental Health Laboratories Men Occupational Health Safety Spermatogenesis Urogenital Diseases Urogenital System
• Keywords:
  Cell-biology Cell-function Cell-metabolism Cell-morphology Cellular-function Cellular-structures Environmental-factors Fertility Laboratory-techniques Reproduction Reproductive-effects Reproductive-system Reproductive-system-disorders Spermatozoa

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• ISSN:
  1472-6483
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Connecticut ; Missouri ; OSHA Region 1 ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  16
• Issue:
  4
• NIOSHTIC Number:
  nn:20034116
• Citation:
  Reprod Biomed Online 2008 Apr; 16(4):570-579
• Contact Point Address:
  The Sperm Physiology Laboratory, Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine. 333 Cedar Street, New Haven, CT 06510
• Federal Fiscal Year:
  2008
• Performing Organization:
  Yale University
• Peer Reviewed:
  True
• Start Date:
  19990930
• Source Full Name:
  Reproductive Biomedicine Online
• End Date:
  20030929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d3d9d410c95396db7d795b1626619a6488c8ecbd96973610e48f351624f2ab8e3259589d8ac77f1d7c4591f823bfce9e2319506ce84c4c63e56cc343db8e80ce
• Download URL:
  https://stacks.cdc.gov/view/cdc/189482/cdc_189482_DS1.pdf
• File Type:
  [PDF - 630.50 KB ]