Procedure for the Quantification of the Biomarker (2-Methoxyethoxy)acetic Acid in Human Urine Samples

Details

• Personal Author:
  B'Hymer, Clayton ; Brown KK ; Butler MA ; Cheever KL
• Description:
  An accurate and precise procedure was developed for the detection and quantification of (2-methoxyethoxy)acetic acid (MEAA), a metabolite and biomarker for human exposure to 2-(2-methoxyethoxy)ethanol. The compound 2-(2-methoxyethoxy)ethanol has a wide array of industrial applications including its use as an additive in military jet fuel. Exposure to 2-(2-methoxyethoxy)ethanol is a health concern owing to its toxicity which includes developmental and teratogenic properties. Sample preparation consisted of liquid-liquid extraction (LLE) and esterification of MEAA to produce the ethyl ester. Measurement was by a gas chromatograph (GC) equipped with a mass selective detector (MSD) using a HP-1 capillary column. Recovery studies of spiked blank urine demonstrated good accuracy and precision; recovery varied between 95 and 103% with relative standard deviations of 8.6% and less. The limit of detection (LOD) for this procedure was found to range from 0.02 to 0.08 ug/ml equivalent levels of MEAA in urine. These data and other aspects of the validation of this procedure will be discussed. [Description provided by NIOSH]
• Subjects:
  Energy Metabolism Esters Ethanol Occupational Health Safety Teratogenesis Urine
• Keywords:
  Acetic Acids Author Keywords: (2-Methoxyethoxy) Acetic Acid Developmental Disorders Ethanols Gas Chromatography Gas Detectors Jet Engine Fuels Metabolites Urinalysis
• ISSN:
  1570-0232
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  145-150
• Volume:
  795
• Issue:
  1
• NIOSHTIC Number:
  nn:20023604
• Citation:
  J Chromatogr B 2003 Sep; 795(1):145-150
• Contact Point Address:
  National Institute for Occupational Safety and Health, Division of Applied Research and Technology, 4676 Columbia Parkway, Cincinnati, OH 45226, USA
• Email:
  cbhymer@cdc.gov
• CAS Registry Number:
  (2-Methoxyethoxy)acetic acid (CAS RN 16024-56-9) ; Diethylene glycol monomethyl ether (CAS RN 111-77-3)
• Federal Fiscal Year:
  2003
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
• End Date:
  20060930
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5a74253a576bf3db4d56cf5d00c584b8e5270f3516afa3522a80104f86276eef5dd9460dc563c743f5db68407331db5bdfd50e231a9cf393c492f34a36e9144f
• Download URL:
  https://stacks.cdc.gov/view/cdc/195943/cdc_195943_DS1.pdf
• File Type:
  [PDF - 124.98 KB ]