Urinary Creatinine Measurement Using a Vitros 250 Chemistry Analyzer Compared with the Jaffe Method

Details

• Personal Author:
  Clark JC ; Kesner JS ; Knecht EA ; Krieg EF
• Description:
  Creatinine forms in muscle as a waste product from creatine and phosphocreatine, and is excreted in urine at relatively constant rates. This allows creatinine excretion to be used as a check for the completeness of 24-hr urine collections, and to normalize, for urine flow rate, urinary concentrations of endogenous and exogenous substances primarily excreted by renal filtration. Because urinary creatinine levels are 10-50 fold higher than those measured in serum, urine samples are typically diluted prior to creatinine analysis. The Vitros 250 chemistry analyzer (Ortho Clinical Diagnostics, Rochester, NY, USA) measures creatinine using a single-slide enzymatic method comprised of a dry, multilayered analytical element coated on a polyester support. The Vitros employs three levels of standard to calibrate creatinine. The highest standard (17 mg/dL) corresponds to 340 mg/dL for urine diluted 20-fold, the dilution recommended by Ortho as a basis for reference accuracy. [Description provided by NIOSH]
• Subjects:
  Mining Occupational Health Safety Ventilation
• Keywords:
  Models Muscles Sampling Sampling Methods Urinalysis Urine Chemistry
• ISSN:
  0009-9147
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  48
• NIOSHTIC Number:
  nn:20023557
• Citation:
  Clin Chem 2002 Jul; 48(Suppl 6):A55
• Federal Fiscal Year:
  2002
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  False
• Source Full Name:
  Clinical Chemistry, International Journal of Laboratory Medicine and Molecular Diagnostics
• Supplement:
  6
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5d5761432c7a90b97bfce73f367c49e65515b8e7b43340f4b180a0500738c6d405f10d715e02f3148831973f76de99b38e79c339817a6823268d8117ff560efd
• Download URL:
  https://stacks.cdc.gov/view/cdc/195918/cdc_195918_DS1.pdf
• File Type:
  [PDF - 527.20 KB ]