Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers

Details

• Personal Author:
  Brandt-Rauf PW ; Li Y-L ; Liu J ; Miao W-B ; Qiu Y-L ; Sun P ; Wang W ; Wu F ; Xia Z-L ; Zhu S-M
• Description:
  OBJECTIVE: To explore the association of the methylation status of MGMT and hMLH1 with chromosome damage induced by vinyl chloride monomer (VCM). MATERIALS AND METHODS: Methylation of MGMT and hMLH1 was measured in 101 VCM-exposed workers by methylation-specific PCR. Chromosome damage in peripheral blood lymphocytes was measured by the cytokinesis-block micronucleus assay. The subjects were divided into chromosome damaged and non-damaged groups based on the normal reference value of micronuclei frequencies determined for two control groups. RESULTS: MGMT promoter methylation was detectable in 5 out of 49 chromosome damaged subjects, but not in the chromosome non-damaged subjects; there was a significant difference in MGMT methylation between the two groups (p < 0.05). CONCLUSIONS: We detected aberrant promoter methylation of MGMT in a small number of chromosome damaged VCM-exposed workers, but not in the chromosome non-damaged subjects. This preliminary observation warrants further investigation in a larger study. [Description provided by NIOSH]
• Subjects:
  Biological Assay Blood Chlorine Chromosomes DNA Energy Metabolism Environmental Monitoring Enzymes Ethylenes Genetics Men Occupational Health Polymers Safety Women

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• Keywords:
  Author Keywords: Vinyl Chloride Monomer Bioassays Blood-cells Chlorides Chromosome-damage Chromosome Damage DNA-damage DNA Methylation Drug-interaction Exposure-assessment Gases Genes HMLH1 Humans Lymphocytes Metabolism MGMT Monomers Polymerization Proteins Tumor-inhibition

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• ISSN:
  1232-1087
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Illinois ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  173-182
• Volume:
  26
• Issue:
  1
• NIOSHTIC Number:
  nn:20043623
• Citation:
  Int J Occup Med Environ Health 2013 Mar; 26(1):173-182
• Contact Point Address:
  Z-L Xia, Department of Occupational Health and Toxicology, School of Public Health, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China
• Email:
  zlxia@shmu.edu.cn
• CAS Registry Number:
  Vinyl chloride (CAS RN 75-01-4)
• Federal Fiscal Year:
  2013
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Illinois at Chicago
• Peer Reviewed:
  True
• Start Date:
  20010701
• Source Full Name:
  International Journal of Occupational Medicine and Environmental Health
• End Date:
  20150831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3f5dfa0f4a394174b890197b812f478b743c425487943190835f7acd3ba6254f0bff67cd242a2441ccc0e315436df5aa235d7894aeebb5dc280fe1f9a5c5fb52
• Download URL:
  https://stacks.cdc.gov/view/cdc/195468/cdc_195468_DS1.pdf
• File Type:
  [PDF - 328.71 KB ]