Effect of Diesel Exhaust Particulate on Bacillus Calmette-Guerin Lung Infection in Mice and Attendant Changes in Lung Interstitial Lymphoid Subpopulations and IFNgamma Response

Details

• Personal Author:
  Bledsoe TA ; Lewis, Daniel M. ; Saxena QB ; Saxena RK ; Simpson JP ; Weissman, David N.
• Description:
  The effect of exposure to diesel exhaust particulate (DEP) on bacillus Calmette-Guerin (BCG) lung infection in mice was studied. C57Bl/6J female mice were infected with BCG (2.5 x 104 bacteria/mouse) by intrapulmonary instillation, with or without coadministration of DEP (100 microg/mouse). Five weeks later, mice exposed to DEP + BCG had about a four-fold higher BCG load in the lungs than mice exposed only to BCG (p < 0.05). DEP treatment alone had no effect on the total number of lung lymphocytes or numbers of T, B, or NK cells recovered from lungs. In contrast, BCG infection significantly increased (p< 0.05) recovery levels of all types of lymphocytes from lungs. Coexposure to DEP + BCG further increased the recovery of lymphocytes from lungs of BCG-infected mice. The pulmonary lymphocyte subpopulation expressing the greatest levels of mRNA for IFNgamma after BCG infection was CD4+ T cells. Expression levels were similar in mice exposed to BCG or BCG + DEP and were elevated as compared to noninfected mice and mice treated with DEP alone. Recovery of IFNgamma-secreting lymphocytes and IFNgamma-secreting T cells was significantly higher (p < 0.05) from lungs of BCG-infected mice as compared to control or DEP-exposed mice. BCG and BCG + DEP groups of mice did not differ significantly in the numbers of IFNgamma-secreting lymphocytes in lungs. Taken together, these results indicated that coexposure to DEP + BCG did not significantly affect the level of IFNgamma response of mice to BCG infection. However, DEP treatment was found to inhibit IFNgamma-induced nitric oxide (NO) production by mouse alveolar macrophages in vitro. Our results indicate that DEP exposure did not alter the IFNgamma response to BCG infection, but reduced responsiveness of alveolar macrophages to IFNgamma. Reduced sensitivity of DEP-exposed alveolar macrophages to IFNgamma may contribute to a greater load of BCG in the lungs of BCG-infected mice given DEP. [Description provided by NIOSH]
• Subjects:
  Air Pollution Animals Blood Immune System Laboratories Lung Lung Diseases Neoplasms Occupational Health Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Air Quality Animal Studies Author Keywords: Exhaust BCG Diesel Macrophages Immune System In-vitro-studies Infection Interferon Laboratory Animals Lung Lung Cancer Lung Disease Lymphocytes Nitric Oxide NK Cells Pulmonary Cancer T Cells

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  66-71
• Volume:
  73
• Issue:
  1
• NIOSHTIC Number:
  nn:20023339
• Citation:
  Toxicol Sci 2003 May; 73(1):66-71
• Contact Point Address:
  NIOSH, HELD, Analytical /services Branch, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  dlewis@cdc.gov
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9)
• Federal Fiscal Year:
  2003
• Peer Reviewed:
  True
• Source Full Name:
  Toxicological Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a47d8a6fa994cfb6e06746dd6515e72f2b12025c2831b6f7bcae7651d4ef86956a4a73c83dd1053cb25f7b60735b6053912fab91b2252c7e0cd92cac69cb8cec
• Download URL:
  https://stacks.cdc.gov/view/cdc/195839/cdc_195839_DS1.pdf
• File Type:
  [PDF - 151.79 KB ]