Expression kinetics of miRNA involved in dermal toluene 2,4-diisocyanate sensitization

Details

• Personal Author:
  Anderson, Stacey E. ; Anderson, Kern E. ; Beezhold K ; Beezhold, Donald H. ; Franko J ; Long C ; Lukomska E ; Meade BJ ; Richardson J
• Description:
  Allergic disease is an important occupational health concern, with work-related asthma and allergic contact dermatitis being the most frequently diagnosed occupational illnesses. Diisocyanates, particularly toluene 2,4-diisocyanate (TDI), have been the leading cause of occupational asthma for many years. Understanding the mechanisms behind allergic disease is critical for treatment and prevention. Recently, the study of post-transcriptional regulation by microRNAs (miRNA) has shed light on mechanisms of allergic disease. The present studies report the expression of miRNA during the sensitization phase of an allergic response to TDI in a murine model. Female BALB/c mice were dermally exposed to TDI (0.1-15% [v/v]) or vehicle. RNA was isolated from superficial parotid lymph nodes at timepoints between 1 h and 15 days post-exposure and then miRNA expression was analyzed using array and real-time quantitative PCR analysis. Consistent changes in miRNA expression were identified for miR-21, miR-22, miR-27b, miR-31, miR-126, miR-155, miR-210, and miR-301a. Following TDI exposure, peak expression was observed by Day 4 for the majority of miRNA evaluated with trends in expression correlated to exposure concentration. Confirmed and predicted targets were identified using Diana-microT, miRanda, miRwalk, and Targetscan algorithms. Evaluation of mRNA expression of cytokine and transcription factor targets suggests that miRNA may have a central role early in TDI sensitization. Understanding the role of these miRNA and their specific mechanism of action in sensitization to TDI may provide pertinent information for the identification of other chemical sensitizers while also contributing to the treatment and prevention of allergic disease. [Description provided by NIOSH]
• Subjects:
  Asthma Asthma, Occupational Biological Assay Dermatitis Environmental Monitoring Hypersensitivity Isocyanates Laboratories Occupational Health Safety Skin Skin Diseases Toluene

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• Keywords:
  Allergic-dermatitis Allergies Author Keywords: Allergic Disease Bioassays Bronchial-asthma Cellular-reactions Contact-allergies Contact-dermatitis Exposure-assessment Exposure-levels Expression Kinetics Kinetics Laboratory-animals Laboratory-testing Lymph-nodes MiRNA Recombinant-DNA Sensitization Skin-sensitivity TDI Chemical Sensitization Toluenes

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• ISSN:
  1547-691X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  250-259
• Volume:
  11
• Issue:
  3
• NIOSHTIC Number:
  nn:20043479
• Citation:
  J Immunotoxicol 2014 Jul-Sep; 11(3):250-259
• Contact Point Address:
  Stacey E. Anderson, PhD, National Institute for Occupational Safety and Health (NIOSH), 1095 Willowdale Drive, Morgantown, WV 26505
• Email:
  sanderson4@cdc.gov
• CAS Registry Number:
  Toluene 2,4-diisocyanate (CAS RN 584-84-9)
• Federal Fiscal Year:
  2014
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Immunotoxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a68ef5253d2810c1533bb1d7f4509f338c68685603c9361ef27c2d8280b14763dfbf502ad649dd1af5bdf222e851f76cf55ac74ae67ac52260b5dc3ea6573d80
• Download URL:
  https://stacks.cdc.gov/view/cdc/195367/cdc_195367_DS1.pdf
• File Type:
  [PDF - 1.78 MB ]