Chronic exposure to carbon nanotubes induces invasion of human mesothelial cells through matrix metalloproteinase-2

Details

• Personal Author:
  Castranova, Vincent ; Dinu CZ ; Liu Y ; Lohcharoenkal W ; Rojanasakul Y ; Stueckle, Todd A. ; Wang L
• Description:
  Malignant mesothelioma is one of the most aggressive forms of cancer known. Recent studies have shown that carbon nanotubes (CNTs) are biopersistent and induce mesothelioma in animals, but the underlying mechanisms are not known. Here, we investigate the effect of long-term exposure to high aspect ratio CNTs on the aggressive behaviors of human pleural mesothelial cells, the primary cellular target of human lung mesothelioma. We show that chronic exposure (4 months) to single- and multiwalled CNTs induced proliferation, migration, and invasion of the cells similar to that observed in asbestos-exposed cells. An up-regulation of several key genes known to be important in cell invasion, notably matrix metalloproteinase-2 (MMP-2), was observed in the exposed mesothelial cells as determined by real-time PCR. Western blot and enzyme activity assays confirmed the increased expression and activity of MMP-2. Whole genome microarray analysis further indicated the importance of MMP-2 in the invasion gene signaling network of the exposed cells. Knockdown of MMP-2 in CNT and asbestos-exposed cells by shRNA-mediated gene silencing effectively inhibited the aggressive phenotypes. This study demonstrates CNT-induced cell invasion and indicates the role of MMP-2 in the process. [Description provided by NIOSH]
• Subjects:
  Asbestos Biological Assay Enzymes Genetics Inorganic Chemicals Lung Lung Diseases Neoplasms Occupational Exposure Occupational Health Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Asbestos Author Keywords: Carbon Nanotubes Bioassays Cancer Cell-alteration Cell-damage Cell-migration Cell Invasion Cellular-reactions Chronic-exposure Enzyme-activity Genes Genotoxic-effects Long-term-exposure Lung-cancer Lung-cells Malignancy Mesothelial-cells Mesothelial Cells Mesothelioma Metalloproteins MMP-2 Nanotechnology Respiratory-system-disorders

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• ISSN:
  1936-0851
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  7
• Issue:
  9
• NIOSHTIC Number:
  nn:20043087
• Citation:
  ACS Nano 2013 Sep; 7(9):7711-7723
• Contact Point Address:
  Yon Rojanasakul, Department of Pharmaceutical Sciences and Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, WV 26506
• Email:
  yrojan@hsc.wvu.edu
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2013
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  ACS Nano
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:df29164fd4c8ab1c161608873a282d11594de381713067be227ea186d23d390358957991e6d82abe87f1706aa74a7db1948ba446f2c28634a7169fe58be76c52
• Download URL:
  https://stacks.cdc.gov/view/cdc/195101/cdc_195101_DS1.pdf
• File Type:
  [PDF - 836.09 KB ]