Multi-walled carbon nanotubes induce human microvascular endothelial cellular effects in an alveolar-capillary co-culture with small airway epithelial cells

Details

• Personal Author:
  Castranova, Vincent ; Guo NL ; Qian Y ; Schwegler-Berry D ; Snyder-Talkington BN
• Description:
  Background: Nanotechnology, particularly the use of multi-walled carbon nanotubes (MWCNT), is a rapidly growing discipline with implications for advancement in a variety of fields. A major route of exposure to MWCNT during both occupational and environmental contact is inhalation. While many studies showed adverse effects to the vascular endothelium upon MWCNT exposure, in vitro results often do not correlate with in vivo effects. This study aimed to determine if an alveolar-capillary co-culture model could determine changes in the vascular endothelium after epithelial exposure to MWCNT. Methods: A co-culture system in which both human small airway epithelial cells and human microvascular endothelial cells were separated by a Transwell membrane so as to resemble an alveolar-capillary interaction was used. Following exposure of the epithelial layer to MWCNT, the effects to the endothelial barrier were determined. Results: Exposure of the epithelial layer to MWCNT induced multiple changes in the endothelial cell barrier, including an increase in reactive oxygen species, actin rearrangement, loss of VE-cadherin at the cell surface, and an increase in endothelial angiogenic ability. Overall increases in secreted VEGFA, sICAM-1, and sVCAM-1 protein levels, as well as increases in intracellular phospho-NF-êB, phospho-Stat3, and phospho-p38 MAPK, were also noted in HMVEC after epithelial exposure. Conclusion: The co-culture system identified that alveolar-capillary exposure to MWCNT induced multiple changes to the underlying endothelium, potentially through cell signaling mediators derived from MWCNT-exposed epithelial cells. Therefore, the co-culture system appears to be a relevant in vitro method to study the pulmonary toxicity of MWCNT. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Environmental Exposure Inhalation Exposure Lung Lung Diseases Men Occupational Health Respiratory System Respiratory Tract Diseases Risk Assessment Risk Factors Safety Women

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• Keywords:
  Airway Epithelium Alveolar-cells Author Keywords: MWCNT Cell-biology Cell-cultures Co-culture Endothelium Environmental-exposure Exposure-levels Humans Inhalants Nanotechnology Pulmonary-disorders Pulmonary-function Pulmonary-system Pulmonary-system-disorders Pulmonary Fibrosis Pulmonary Inflammation Risk-factors

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• ISSN:
  1743-8977
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  35
• Volume:
  10
• NIOSHTIC Number:
  nn:20043071
• Citation:
  Part Fibre Toxicol 2013 Aug; 10:35
• Contact Point Address:
  Brandi N Snyder-Talkington, Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505-2888
• Email:
  yaq2@cdc.gov
• Federal Fiscal Year:
  2013
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Particle and Fibre Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:07b71503881bd6daa2966aff6094798b7a6227e1b5a94a0aa39bb3270cc14a3a3ef2401355dde77e4bd92b6700ab308b7aa576239e66c73e98c63b95bde4a646
• Download URL:
  https://stacks.cdc.gov/view/cdc/195089/cdc_195089_DS1.pdf
• File Type:
  [PDF - 3.59 MB ]