U.S. flag An official website of the United States government.
Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

i

Band acceleration device for enhanced selectivity with tandem-column gas chromatography



Details

  • Personal Author:
  • Description:
    An electrically heated and air cooled metal sheath surrounding the first 50 cm of the second column in a series-coupled, capillary-column ensemble of a non-polar and a polar column is used to obtain enhanced isothermal separation of component pairs that are separated by the first column in the ensemble but co-elute from the ensemble by virtue of the different selectivity of the two columns. As the first of the two components passes into the second column, a current pulsed through the metal sheath rapidly heats the first 50 cm of the second column thus accelerating the band for the first component. Ensemble retention-time shifts of several seconds are easily obtained. The device is then rapidly cooled to quiescent oven temperature by a flow of pressured air through the space between the metal sheath and the fused silica capillary column and an additional flowthrough a larger, co-axial plastic tube. Both heating and cooling require only a fewseconds. If substantial cooling of the device occurs before the band for the second component enters the device, the band experiences less thermally-induced acceleration with the result that the separation of the two targeted components is enhanced in the ensemble chromatogram with no significant change in the pattern of peaks for the other mixture components. If the device is cooled to a temperature below oven temperature before the arrival of the band for the second component, this band will be slowed, and further enhancement of separation is achieved in the ensemble chromatogram. A band trajectory model, based on retention factor versus temperature data for the two components in the two columns, is used to predict peak separation and to aid in the selection of temperature-pulse initiation times. [Description provided by NIOSH]
  • Subjects:
  • Keywords:
  • ISSN:
    0021-9673
  • Document Type:
    Text
  • Funding:
    Grant
  • Genre:
    Journal Article
  • Place as Subject:
  • CIO:
    National Institute for Occupational Safety and Health (NIOSH)
  • Topic:
    Workplace Safety & Health
  • Location:
    North America
  • Pages in Document:
    151-159
  • Volume:
    1086
  • Issue:
    1
  • NIOSHTIC Number:
    nn:20042740
  • Citation:
    J Chromatogr A 2005 Sep; 1086(1-2):151-159
  • Email:
    rdsacks@umich.edu
  • Federal Fiscal Year:
    2005
  • Performing Organization:
    University of Michigan, Ann Arbor
  • Peer Reviewed:
    True
  • Start Date:
    20060601
  • Source Full Name:
    Journal of Chromatography A
  • End Date:
    20160430
  • Collection(s):
  • Main Document Checksum:
    urn:sha-512:6f1e1a3476c0c2d4b545515ad537694b6100cbff6c61c2848ee3f95a63a97c20b6009164fc39a34d3b3adc4a6d50d900f849b6a4e2ce9cc9f5227219d0ca8a3e
  • Download URL:
  • File Type:
    Filetype[PDF - 335.25 KB ]
PREVIOUS
ON THIS PAGE
Details
CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners.

As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.
BACK TO TOP