Effect of pulmonary exposure to welding fumes on cardiomyocyte contractility

Details

• Personal Author:
  Antonini JM ; Castranova, Vincent ; Kan H ; Popstojanov R ; Salmen R ; Ye M ; Zheng W
• Description:
  Studies have found that pulmonary exposure to welding fumes is positively associated with a higher incidence of cardiovascular events. We reported previously that pulmonary exposure to welding fumes, manual metal arc-hard surfacing (MMAHS), has a negative impact on cardiac function as evidenced by reduced heart contractility. However, the mechanisms underlying MMA-HS-induced depression of cardiac contractility remain unclear. To study the mechanisms, rats were given an intratracheal instillation of MMA-HS welding fumes (2 mg/rat) or saline once a week for seven weeks. Cardiomyocytes were isolated at 1 and 7 days post-exposure. Cardiomyocyte contractility and intracellular calcium level in response to increasing concentrations of adrenoreceptor agonist isoprenaline and extracellular calcium were assessed using a Myocyte Calcium Imaging/Cell Length System. Pulmonary exposure to MMA-HS blunted contractile function in response to both isoprenaline and calcium at 1 day post-exposure (P < 0.01; P < 0.05, respectively). A blunted contractile response was also observed with welding fume treated rats at 7 days post-exposure in response to isoprenaline and calcium (P < 0.01). Intracellular calcium level in response to extracellular calcium stimulation was reduced at 7 days post-exposure (P < 0.05). These findings suggest that pulmonary exposure to welding fumes impairs cardiac function by decreasing cardiomyocyte contractility through a defect in the adrenergic signaling pathway and intracellular calcium handling. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Calcium Compounds Cardiovascular Diseases Cardiovascular System Environmental Monitoring Laboratories Lung Metals Occupational Health Respiratory System Safety Toxicology Welding

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• Keywords:
  Arc-welders Arc-welding Calcium-compounds Cardiac-function Cardiopulmonary-function Cardiovascular-function Cardiovascular-system Cardiovascular-system-disorders Cell-alteration Cell-function Cellular-function Cellular-reactions Dose-response Exposure-assessment Exposure-levels Fumes Heart Laboratory-animals Metal-fumes Muscle-contraction Pulmonary-system Welders Welding-industry

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; OSHA Region 6 ; Texas ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  132
• Issue:
  1
• NIOSHTIC Number:
  nn:20042411
• Citation:
  Toxicologist 2013 Mar; 132(1):242
• CAS Registry Number:
  Calcium (CAS RN 7440-70-2)
• Federal Fiscal Year:
  2013
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 52nd Annual Meeting and ToxExpo, March 10-14, 2013, San Antonio, Texas
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:876d7ce0dbdf751c78da9af55b73ca45f2ea9435ffbe967bd41df9d90dda587094553779554ac52f940756faf04f25c23a8a4135731e4c4080ce9ad3cb37e12a
• Download URL:
  https://stacks.cdc.gov/view/cdc/194643/cdc_194643_DS1.pdf
• File Type:
  [PDF - 574.93 KB ]