Alcohol exposure alters mouse lung inflammation in response to inhaled dust

Details

• Personal Author:
  Bailey KL ; Boten J ; DeVasure J ; McCaskill ML ; Poole JA ; Romberger DJ ; Sisson JH ; Wyatt TA
• Description:
  Alcohol exposure is associated with increased lung infections and decreased mucociliary clearance. Occupational workers exposed to dusts from concentrated animal feeding operations (CAFOs) are at risk for developing chronic inflammatory lung diseases. Agricultural worker co-exposure to alcohol and organic dust has been established, although little research has been conducted on the combination effects of alcohol and organic dusts on the lung. Previously, we have shown in a mouse model that exposure to hog dust extract (HDE) collected from a CAFO results in the activation of protein kinase C (PKC), elevated lavage fluid cytokines/chemokines including interleukin-6 (IL-6), and the development of significant lung pathology. Because alcohol blocks airway epithelial cell release of IL-6 in vitro, we hypothesized that alcohol exposure would alter mouse lung inflammatory responses to HDE. To test this hypothesis, C57BL/6 mice were fed 20% alcohol or water ad libitum for 6 weeks and treated with 12.5% HDE by intranasal inhalation method daily during the final three weeks. Bronchoalveolar lavage fluid (BALF), tracheas and lungs were collected. HDE stimulated a 2-4 fold increase in lung and tracheal PKCe (epsilon) activity in mice, but no such increase in PKCe activity was observed in dust-exposed mice fed alcohol. Similarly, alcohol-fed mice demonstrated significantly less IL-6 in lung lavage in response to dust than that observed in control mice instilled with HDE. TNFa levels were also inhibited in the alcohol and HDE-exposed mouse lung tissue as compared to the HDE only exposed group. HDE-induced lung inflammatory aggregates clearly present in the tissue from HDE only exposed animals were not visually detectable in the HDE/alcohol co-exposure group. Statistically significant weight reductions and 20% mortality were also observed in the mice co-exposed to HDE and alcohol. These data suggest that alcohol exposure depresses the ability of the lung to activate PKCe-dependent inflammatory pathways to environmental dust exposure. These data also define alcohol as an important co-exposure agent to consider in the study of inhalation injury responses. [Description provided by NIOSH]
• Subjects:
  Agriculture Dust Laboratories Lung Occupational Health Pathology Respiratory System Safety
• Keywords:
  Agricultural-workers Alcohols Author Keywords: Alcohol Dust-exposure Dusts Inflammation Laboratory-animals Lung-tissue Mortality Mucous-membranes Organic-dusts Organic Dust

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• ISSN:
  2072-6643
• Document Type:
  Text
• Funding:
  Grant ; Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Nebraska ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  4
• Issue:
  7
• NIOSHTIC Number:
  nn:20041902
• Citation:
  Nutrients 2012 Jul; 4(7):695-710
• Contact Point Address:
  Todd A. Wyatt, Department of Environmental, Agricultural, and Occupational Health, College of Public Health, University of Nebraska Medical Center, Omaha, NE 68198
• Email:
  twyatt@unmc.edu
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center, Omaha, Nebraska
• Peer Reviewed:
  True
• Start Date:
  20060801
• Source Full Name:
  Nutrients
• End Date:
  20160731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8225ea382336cb370a597689ae04f42223267c838941109ccfb94a70773104275ce3c99078a856926cac28538936fb13856934c5d8aba4ed2d7d32385c0f08ec
• Download URL:
  https://stacks.cdc.gov/view/cdc/194325/cdc_194325_DS1.pdf
• File Type:
  [PDF - 1.50 MB ]