Characterization of endotoxin collected on California dairies using personal and area-based sampling methods

Details

• Personal Author:
  Bennett DH ; Dooley GP ; Garcia J ; Mehaffy J ; Mitchell DC ; Mitloehner FM ; Reynolds SJ ; Schenker MB ; Silva R ; Tancredi DJ
• Description:
  Endotoxin, found in the cell wall of gram negative bacteria, is an important contributor to the biological activity of agriculture particulate matter (PM). We analyzed endotoxin in PM collected on 13 California dairies and from the breathing zone of 226 workers during the summer months of 2008. Two particle size fractions were measured: PM2.5 and inhalable PM. Recombinant factor C assays were used to analyze biologically active endotoxin, while gas chromatography coupled with mass spectrometry in tandem was used to quantify total lipopolysaccharide. Biologically active endotoxin concentrations in the inhalable PM size fraction from area-based samples ranged from 11-2095 EU/m3 and from 45-2061 EU/m3 for personal samples. Total endotoxin in the inhalable PM size fraction ranged from 75-10,166 pmol/m3 for area-based samples and 34-11,689 pmol/m3 for personal samples. Area-based geometric mean concentrations for biologically active endotoxin and total endotoxin in PM2.5 and inhalable PM size fractions were 3 EU/m3, 149 EU/m3, 60 pmol/m3, and 515 pmol/m3, respectively. Personal geometric mean concentrations in the inhalable PM size fraction were 334 EU/m3, and 1178 pmol/m3. Biologically active and total endotoxin concentration variation was best explained by meteorological data, wind speed, relative humidity, and dairy waste management practices. Differences in endotoxin concentration and composition were found across locations on the dairy. [Description provided by NIOSH]
• Subjects:
  Agriculture Endotoxins Farmers Lipids Occupational Health Particulate Matter Safety
• Keywords:
  Author Keywords: 3-OHFA Bacteria Breathing Zone Dairy Endotoxin Farm Workers Gas Chromatography Lipopolysaccharide Mass Spectrometry Particulate Matter Particulates Sampling

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• ISSN:
  1545-9624
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; Colorado ; OSHA Region 8 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  9
• Issue:
  10
• NIOSHTIC Number:
  nn:20041824
• Citation:
  J Occup Environ Hyg 2012 Oct; 9(10):580-591
• Contact Point Address:
  Deborah H. Bennett, Department of Public Health Sciences, University of California, Davis, One Shields Avenue, Davis, CA 95616
• Email:
  dhbennett@ucdavis.edu
• Federal Fiscal Year:
  2013
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Journal of Occupational and Environmental Hygiene
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ba7cfc36dfdb08da8272ddfc667e6ae0db557091644d4b796ce6656d074daca87b4737f0dc27824264fac2567420b25bce0e69cd26d4566e2af3a234c6898996
• Download URL:
  https://stacks.cdc.gov/view/cdc/194277/cdc_194277_DS1.pdf
• File Type:
  [PDF - 371.67 KB ]