Expression pattern of oxidative stress and antioxidant defense-related genes in the aging Fischer 344/NHsd rat cochlea

Details

• Personal Author:
  Chen G-D ; Coling DE ; Henderson D ; Hu BH ; Manohar S ; Salvi R ; Tanaka C
• Description:
  The biological mechanisms that give rise to age-related hearing loss (ARHL) are still poorly understood. However, there is growing recognition that oxidative stress may be an important factor. To address this issue, we measured the changes in the expression of cochlear oxidative stress and antioxidant defense-related genes in young (2 months old), middle-aged (12 months old), and old (21-25 months old) Fischer 344/NHsd (F344/NHsd) rats and compared gene expression changes with ARHL. A quantitative real-time reverse transcription polymerase chain reaction array revealed a significant age-related downregulation of only 1 gene, stearoyl-coenzyme A desaturase 1, and upregulation of 12 genes: 24-dehydrocholesterol reductase; aminoadipate-semialdehyde synthase; cytoglobin; dual oxidase 2; glutathione peroxidase 3; glutathione peroxidase 6; glutathione S-transferase, kappa 1; glutathione reductase; nicotinamide adenine dinucleotide phosphate (NAD(P)H) dehydrogenase, quinone 1; solute carrier Family 38, Member 5; thioredoxin interacting protein; and vimentin. Statistical analyses revealed significant correlations between gene expression and auditory function in 8 genes. Our results identified specific subsets of oxidative stress genes that appear to play an important role in ARHL in the Fischer 344/NHsd rat. [Description provided by NIOSH]
• Subjects:
  Age Factors Aldehydes Amino Acids Antioxidants Ear Energy Metabolism Enzymes Formaldehyde Genetics Hearing Loss Histocytochemistry Laboratories Nucleotides Occupational Health Phosphoric Acids Quinones Safety Sense Organs

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• Keywords:
  Adipates Age-factors Amino-acids Auditory-system Cytochemistry Ears Enzyme-activity Genes Hearing-loss Laboratory-animals Laboratory-testing Metabolism Oxidative-enzymes Oxidative-processes Peroxidases Phosphates Physiological-response Physiology Protein-chemistry Proteins Thiones

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• ISSN:
  0197-4580
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; Oregon ; OSHA Region 10 ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  33
• Issue:
  8
• NIOSHTIC Number:
  nn:20041721
• Citation:
  Neurobiol Aging 2012 Aug; 33(8):1842.e1-1842.e14
• Contact Point Address:
  Chiemi Tanaka, Oregon Hearing Research Center, Department of Otolaryngology, Mail Code NRC04, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
• Email:
  tanakach@ohsu.edu
• Federal Fiscal Year:
  2012
• Performing Organization:
  State University of New York at Buffalo
• Peer Reviewed:
  True
• Start Date:
  20050501
• Source Full Name:
  Neurobiology of Aging
• End Date:
  20090430
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c923ab0b29a461531dddba611003c8fca22178962f50239eb51ea48c58b610c202196b574ab2c524a48980d122a511dbb87e8cf78f4ab23f1929b1def80a9eec
• Download URL:
  https://stacks.cdc.gov/view/cdc/194209/cdc_194209_DS1.pdf
• File Type:
  [PDF - 324.58 KB ]