Single cell gel electrophoresis assay (comet assay) for evaluating nanoparticles-induced DNA damage in cells

Details

• Personal Author:
  Bowman L ; Castranova, Vincent ; Ding M
• Description:
  Comet assay provides a rapid and reliable way to screen genotoxic effects of a wide variety of nanoparticles. Single cell gel electrophoresis assay (SCGE) or comet assay gained in popularity as a standard technique and is widely used for testing novel particles or chemicals for genotoxicity, monitoring environmental contamination with genotoxins, human biomonitoring and molecular epidemiology, and fundamental research in DNA damage and repair. Here, we describe the method of alkaline comet assay to detect nanoparticle-induced DNA damage. [Description provided by NIOSH]
• Subjects:
  Alkalies Chemistry DNA Occupational Health Particulate Matter Safety
• Keywords:
  Author Keywords: Nanoparticles Cell-biology Cell-function Chemical-composition Comet Assay DNA-damage DNA Damage Genotoxicity Nanotechnology Particulates Reactive Oxygen Species

  More +
• ISBN:
  9781617799525
• Publisher:
  Springer
• Document Type:
  Text
• Genre:
  Book
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  415-422
• Volume:
  906
• NIOSHTIC Number:
  nn:20041170
• Citation:
  Nanoparticles in biology and medicine: methods and protocols, methods in molecular biology. Soloviev M, ed. New York: Springer, 2012 Jan; 906:415-422
• Editor(s):
  Soloviev M
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Construction ; Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  Nanoparticles in biology and medicine: methods and protocols, methods in molecular biology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5f5b037e8dc4c45cb81357e34244a09162fa92e3886b6b2a14e58b1d31bf554c476b9777698d621d44b024b6b2936e672874726852497c177fa7b204cc64305a
• Download URL:
  https://stacks.cdc.gov/view/cdc/193786/cdc_193786_DS1.pdf
• File Type:
  [PDF - 267.97 KB ]