Detection of DNA damage in workers exposed to JP-8 jet fuel

Details

• Personal Author:
  B'Hymer, Clayton ; Butler MA ; Clark JC ; Gibson RL ; Krieg EF Jr. ; Marlow, Kate L. ; Mathias PI ; Singh NP ; Toennis CA ; B'Hymer, Clayton ; Butler MA ; Clark JC ; Gibson RL ; Krieg EF Jr. ; Marlow, Kate L. ; Mathias PI ; Singh NP ; Toennis CA
• Description:
  The genotoxicity of jet propulsion fuel 8 (JP-8) was assessed in the leukocytes of archived blood specimens from U.S. Air Force personnel using the comet assay. No differences in mean comet assay measurements were found between low, moderate, and high exposure groups before or after a 4 hour work shift. Before the work shift, mean tail DNA and mean tail (Olive) moment increased as the concentration of benzene measured in end-exhaled breath increased, indicating that prior environmental or work-related exposures to benzene produced DNA damage. The number of cells with highly damaged DNA decreased as the pre-shift benzene concentration in breath increased. It is not clear why the decrease is occurring. Mean tail DNA and mean tail (Olive) moment decreased as the concentrations of benzene and naphthalene measured in breath immediately after the work shift increased. These inverse relationships may reflect a slower rate of absorption or a faster rate of expiration of benzene in the lung. The number of cells with highly damaged DNA increased as the concentration of urinary (2-methoxyethoxy)acetic acid (MEAA) increased. This relationship was not seen in urinary MEAA adjusted for creatinine. MEAA is a metabolite of the deicing agent 2-(2-methoxyethoxy)ethanol contained in JP-8. MEAA or a component of JP-8 correlated with MEAA may have a toxic effect on DNA. [Description provided by NIOSH]
• Subjects:
  Acetamides Aircraft Benzene Biological Assay Blood Chemistry DNA Environmental Monitoring Ethanol Fuel Oils Hematologic Tests Naphthalenes Occupational Exposure Occupational Health Respiratory System Safety

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• Keywords:
  Absorption-rates Acetic-acids Benzenes Bioassays Blood-samples Breathing Cell-damage Chemical-indicators Deoxyribonucleic-acids DNA-damage Employee-exposure Ethanols Exposure-assessment Fuels Genotoxic-effects Genotoxicity Jet-engine-fuels Leukocytes Military-personnel

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• ISSN:
  1383-5718
• Document Type:
  Text
• Genre:
  Journal Article ; Journal Article
• Place as Subject:
  Maryland ; Ohio ; OSHA Region 10 ; OSHA Region 3 ; OSHA Region 5 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology ; DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Pages in Document:
  218-227
• Volume:
  747
• Issue:
  2
• NIOSHTIC Number:
  nn:20040849
• Citation:
  Mutat Res Genet Toxicol Environ Mutagen 2012 Sep; 747(2):218-227
• Contact Point Address:
  Edward F. Krieg Jr., National Institute for Occupational Safety and Health, Division of Applied Research and Technology, MS C-22, 4676 Columbia Parkway, Cincinnati, OH 45226, USA
• Email:
  EKrieg@cdc.gov
• CAS Registry Number:
  (2-Methoxyethoxy)acetic acid (CAS RN 16024-56-9) ; Benzene (CAS RN 71-43-2) ; Diethylene glycol monomethyl ether (CAS RN 111-77-3) ; Naphthalene (CAS RN 91-20-3) ; (2-Methoxyethoxy)acetic acid (CAS RN 16024-56-9) ; Benzene (CAS RN 71-43-2) ; Diethylene glycol monomethyl ether (CAS RN 111-77-3) ; Naphthalene (CAS RN 91-20-3)
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Healthcare and Social Assistance ; Services ; Healthcare and Social Assistance ; Services
• Peer Reviewed:
  True
• Source Full Name:
  Mutation Research - Genetic Toxicology and Environmental Mutagenesis
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4c7145544caea336445daf52711f4beea27ddb1ebad2899705f0705fed8a70e0335005cfeb90c1ff86f33f2151314869bdd3f28366dcc0b548301f257818e8d9
• Download URL:
  https://stacks.cdc.gov/view/cdc/193568/cdc_193568_DS1.pdf
• File Type:
  [PDF - 329.81 KB ]