A small whole-body exposure chamber for laboratory use

Details

• Personal Author:
  Achutan, Chandran ; O'Neill ME ; O'Shaughnessy PT ; Thorne PS
• Description:
  With the development of transgenic and specialized mouse strains, there is an increased need for inhalation exposure systems designed for smaller exposure groups. An inhalation exposure chamber, designed specifically for the exposure of up to 40 mice, was characterized. The chamber was fabricated from 0.32-cm-thick ((1)/(8)-in) aluminum sheets with outside dimensions of 61 cm long by 32 cm high by 34 cm deep, resulting in an internal volume of 65 L. Two stainless-steel open-mesh cages, separated by an absorbent barrier, can be stacked within the central portion of the chamber. Access is provided through a gasketed door with a safety-glass face. Tests were performed to determine the chamber leakage rate, degree of mixing, and spatial variation of two aerosols within the chamber. Results indicated that the fractional leakage rate was 0.0003 min(-1), well below a reported criterion for an operating chamber. Chamber operation gave similar mixing performance with, or without, use of an interior fan. For aerosols with a mass median aerodynamic diameter (MMAD) of 2.56 micro m and 3.14 micro m, the spatial variation of particulate matter concentration resulted in coefficients of variation (CVs) of 4.8% and 11.0%, respectively. These CV values are comparable to those obtained from similar studies involving other inhalation exposure chambers. [Description provided by NIOSH]
• Subjects:
  Aerosols Laboratories Occupational Health Particulate Matter Respiratory System Safety
• Keywords:
  Aerosol-particles Equipment-design Equipment-reliability Exposure-chambers Inhalation-studies Laboratory-animals Particulate-dust Particulates Testing-equipment
• ISSN:
  0895-8378
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Iowa ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  251-263
• Volume:
  15
• Issue:
  3
• NIOSHTIC Number:
  nn:20040450
• Citation:
  Inhal Toxicol 2003 Mar; 15(3):251-263
• Contact Point Address:
  Patrick T. O'Shaughnessy, Department of Occupational and Environmental Health, University of Iowa, 100 Oakdale Campus, #137 IREH, Iowa City, IA 52242-5000
• Email:
  patrick-oshaughnessy@uiowa.edu
• Federal Fiscal Year:
  2003
• Performing Organization:
  University of Iowa
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Inhalation Toxicology
• End Date:
  20290630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a89784173d1bb76d6ef62d387ed73048e11328b6efd4f970dd6ec2d779330d136ef38e310e73aa86287aebd9d394272d586c60f0ccfc3d3d4ceb9c89e7558cc2
• Download URL:
  https://stacks.cdc.gov/view/cdc/193305/cdc_193305_DS1.pdf
• File Type:
  [PDF - 650.16 KB ]