Evaluation of the interactions between multiwalled carbon nanotubes and Caco-2 cells

Details

• Personal Author:
  Bressler JP ; Clark KA ; Cooke CA ; Fairbrother DH ; Lees PSJ ; O'Driscoll C ; Smith BA ; Wepasnick K
• Description:
  The aim of this study was to determine whether multiwalled carbon nanotubes (MWNCT) are taken up by and are toxic to human intestinal enterocytes using the Caco-2 cell model. Caco-2 cells were exposed to 50 µg/ml MWCNT (oxidized or pristine) for 24 h, and experiments were repeated in the presence of 2.5 mg/L natural organic matter. Cells displayed many of the properties that characterize enterocytes, such as apical microvilli, basolateral basement membrane, and glycogen. The cell monolayers also displayed tight junctions and electrical resistance. Exposure to pristine and oxidized MWCNT, with or without natural organic matter, did not markedly affect viability, which was assessed by measuring activity of released lactate dehydrogenase (LDH) and staining with propidium iodide. Ultrastructural analysis revealed some damage to microvilli colocalized with the MWCNT; however, neither type of MWCNT was taken up by Caco-2 cells. In contrast, pristine and oxidized MWCNT were taken up by the macrophage RAW 264.7 line. Our study suggests that intestinal enterocytes cells do not take up MWCNT. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Cytotoxins Energy Metabolism Environmental Monitoring Hazardous Substances Occupational Health Safety
• Keywords:
  Cell-alteration Cell-cultures Cell-metabolism Cellular-reactions Cytotoxic-effects Drug-interaction Exposure-assessment Exposure-levels Exposure-methods Humans Intestinal-cells Membrane-dysfunction Metabolism Nanotechnology Organic-compounds Oxidation Toxic-effects Ultrastructure

  More +
• ISSN:
  1528-7394
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Maryland ; OSHA Region 3
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  25-35
• Volume:
  75
• Issue:
  1
• NIOSHTIC Number:
  nn:20040114
• Citation:
  J Toxicol Environ Health A 2012 Jan; 75(1):25-35
• Contact Point Address:
  Joseph P. Bressler, Kennedy Krieger Institute, 707 North Broadway, Baltimore MD 21215, USA
• Email:
  bressler@kennedykrieger.org
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0) ; Propidium iodide (CAS RN 25535-16-4)
• Federal Fiscal Year:
  2012
• Performing Organization:
  Johns Hopkins University
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Journal of Toxicology and Environmental Health, Part A: Current Issues
• End Date:
  20280630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5351f6e51028f6f3383a75b308718f47302d45ddac752334c19e4cfae6589befef9e62a98efd0a8c7c1a06ea5d93b0c66b1308d4f148de39304750513167d4b0
• Download URL:
  https://stacks.cdc.gov/view/cdc/193086/cdc_193086_DS1.pdf
• File Type:
  [PDF - 1.08 MB ]