Global phospholipidomics analysis reveals selective pulmonary peroxidation profiles upon inhalation of single-walled carbon nanotubes

Details

• Personal Author:
  Amoscato AA ; Fadeel B ; Kagan VE ; Kapralova VI ; Kisin ER ; Lahesmaa R ; Murray A ; Samhan-Arias AK ; Shvedova AA ; Sparvero LJ ; Swedin L ; Tyurin VA ; Tyurina YY
• Description:
  It is commonly believed that nano materials cause nonspecific oxidative damage. Our mass spectrometry-based oxidative lipidomics analysis of all major phospholipid classes revealed highly selective patterns of pulmonary peroxidation after inhalation exposure of mice to single-walled carbon nanotubes. No oxidized molecular species were found in the two most abundant phospholipid classes: phosphatidylcholine and phosphatidylethanolamine. Peroxidation products were identified in three relatively minor classes of anionic phospholipids, cardiolipin, phosphatidylserine, and phosphatidylinositol, whereby oxygenation of polyunsaturated fatty acid residues also showed unusual substrate specificity. This nonrandom peroxidation coincided with the accumulation of apoptotic cells in the lung. A similar selective phospholipid peroxidation profile was detected upon incubation of a mixture of total lung lipids with H(2)O(2)/cytochrome c known to catalyze cardiolipin and phosphatidylserine peroxidation in apoptotic cells. The characterized specific phospholipid peroxidation signaling pathways indicate new approaches to the development of mitochondria-targeted regulators of cardiolipin peroxidation to protect against deleterious effects of pro-apoptotic effects of single-walled carbon nanotubes in the lung. [Description provided by NIOSH]
• Subjects:
  Animals Environmental Monitoring Laboratories Lipids Lung Mass Spectrometry Occupational Health Respiratory System Safety
• Keywords:
  Animal-studies Apoptosis Author Keywords: Single-walled Carbon Nanotubes Cardiolipin Oxidation Cell-function Cellular-reactions Exposure-assessment Exposure-levels Exposure-methods Fatty-acids Inhalation-studies Laboratory-animals Laboratory-testing Lipid-peroxidation Lipidomics Lung-cells Mass-spectrometry Mouse Lung Nanotechnology Nanotubes Oxidative-phosphorylation Oxidative-processes Oxidative Lipidomics Peroxidases Phosphatidylserine Oxidation Phospholipids Pulmonary-system

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• ISSN:
  1936-0851
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  5
• Issue:
  9
• NIOSHTIC Number:
  nn:20039792
• Citation:
  ACS Nano 2011 Sep; 5(9):7342-7353
• Contact Point Address:
  Valerian E. Kagan, University of Pittsburgh, Center for Free Radical and Antioxidant Health, Pittsburgh, PA 15260 USA
• Email:
  kagan@pitt.edu
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Manufacturing ; Mining
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  ACS Nano
• End Date:
  20160630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:722e7e7ad8796821af2d4babca4530201ab8f404b639f2016975c28f6bdedbdc4c01cf98fdfebbe812fa7bb63dd5e8bd4353867c412fef839893fd9cd88197e7
• Download URL:
  https://stacks.cdc.gov/view/cdc/192889/cdc_192889_DS1.pdf
• File Type:
  [PDF - 1.12 MB ]