Spectroelectrochemical sensing of pyrene metabolites 1-hydroxypyrene and 1-hydroxypyrene-glucuronide

Details

• Personal Author:
  Heineman WR ; Seliskar CJ ; Talaska G ; Wilson RA
• Description:
  Spectroelectrochemical sensing in an optically transparent thin layer electrode (OTTLE) cell was used for detecting the polycyclic aromatic hydrocarbon (PAH) biomarkers 1-hydroxypyrene (1-pyOH) and 1-hydroxypyrene-glucuronide (1-pyOglu) in phosphate buffer and artificial urine. This approach uses selective electrochemical modulation of a fluorescence signal by sequentially oxidizing the analytes in an OTTLE cell to distinguish between their overlapping fluorescence spectra. This technique allows for complete oxidation and signal modulation in approximately 15 min for each analyte; a mixture of 1-pyOH and its glucuronic acid conjugate can be analyzed in 30 min. Calibration curves consisting of the fluorescence change vs analyte concentration for 1-pyOH and 1-pyOglu yielded linear ranges from 10 nM to 1 µM and from 1 nM to 1 µM, respectively. With the use of these results, the calculated limits of detection were determined to be 1 × 10(-8) M for 1-pyOH and 9 × 10(-11) M for 1-pyOglu. [Description provided by NIOSH]
• Subjects:
  Biomarkers Occupational Health Safety
• Keywords:
  Cell-biology Cell-function Cellular-reactions Electrochemical-analysis Mathematical-models Physical-reactions Physiological-effects Physiological-response Statistical-analysis
• ISSN:
  0003-2700
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  83
• Issue:
  10
• NIOSHTIC Number:
  nn:20039537
• Citation:
  Anal Chem 2011 May; 83(10):3725-3729
• Contact Point Address:
  William R. Heineman, Department of Chemistry, University of Cincinnati, 301 Clifton Court, Cincinnati, Ohio 45221-0172
• Email:
  william.heineman@uc.edu
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  True
• Start Date:
  20090901
• Source Full Name:
  Analytical Chemistry
• End Date:
  20120831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:aae266c836f8e5a82f679b29f71a627e2f89103e79582d0892fc741e07fa31813015e25ae945689af78cc4dcf55061be0df37fe973a95c2817d8ceb3ee787ac1
• Download URL:
  https://stacks.cdc.gov/view/cdc/192739/cdc_192739_DS1.pdf
• File Type:
  [PDF - 1.30 MB ]