Determination of low molecular weight aldehydes and ketones by high performance liquid chromatography

Details

• Personal Author:
  Que Hee-S ; Wiesenthal K
• Description:
  Human activities contribute to the production of low molecular weight aldehydes and ketones in air and water. They are released in to air as byproducts of combustion processes and industrial uses. Aldehydes and ketones are formed as byproducts of ozone reaction during water treatment processes. They are irritants; formaldehyde, acetaldhyde, and crotonaldehyde are carcinogens. While there is a gas chr0- matography method, there is no high performance liquid chromatography (HPLC) method for these derivatives. nus was the aim of the present study. O-(2,3,4,5,6-pentafluorobenyl)hydroxylamine hydrochloride (PFBHA) is a derivatizing agent for the carbonyl compound to produce oxime derivatives. The oximes were extracted with hexane, the solvent evaporated, and then dissolved in acetronitrile. Eleven different oxime derivatives were mixed in a cocktaiL They were resolved on a 25-cm length BioSil ODS-5S reverse phase of film thickness 5 um column, with the following conditions: mobile phase 57% acetonitrile and 43% water; temperature 39 degrees C; flow rate 0.8 mL/min; UV detector wavelength 22 nm. These conditions allowed baseline resolution of 11 oximes. The run time was 113 minutes. The detection limit for most derivatives was 1 mg/L. The linear region for most derivatives was 1 mg/L to 36 mg/L. HPLC can be used to complement the standard GC method for quality control and quality assurance. It is inexpensive and easily performed. [Description provided by NIOSH]
• Subjects:
  Chromatography Environmental Exposure Environmental Monitoring Laboratories Occupational Health Safety Solvents Spectrum Analysis
• Keywords:
  Air-monitoring Air-samplers Air-sampling Air-sampling-equipment Air-sampling-techniques Chromatographic-analysis Environmental-exposure Laboratory-techniques Laboratory-testing Membrane-filters Quantitative-analysis Sampling Sampling-methods Spectrographic-analysis Vapors

  More +
• Publisher:
  American Industrial Hygiene Association
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  51
• NIOSHTIC Number:
  nn:20039199
• Citation:
  American Industrial Hygiene Conference and Exposition, May 19-23, 1997, Dallas, Texas. Fairfax, VA: American Industrial Hygiene Association, 1997 May; :51
• Federal Fiscal Year:
  1997
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Performing Organization:
  University of California Los Angeles, Los Angeles, CA
• Peer Reviewed:
  False
• Start Date:
  19950901
• Source Full Name:
  American Industrial Hygiene Conference and Exposition, May 19-23, 1997, Dallas, Texas
• End Date:
  19990831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a794d84b15572fa2fa32e537835374f3462eaad7385c7b427e0236eda9d4048898bb93a7537ee2f5a33c88b768d8c8e54b6dc611a76f821f36b00273c5c56e61
• Download URL:
  https://stacks.cdc.gov/view/cdc/192636/cdc_192636_DS1.pdf
• File Type:
  [PDF - 881.18 KB ]