Activation of MAP kinases by hexavalent chromium, manganese and nickel in human lung epithelial cells

Details

• Personal Author:
  Pascal LE ; Tessier DM
• Description:
  Epidemiological studies indicate that workers who perform welding operations are at increased risk for bronchitis, siderosis, occupational asthma and lung cancer due to fume exposure. Welding fumes are a complex chemical mixture, and the metal composition is hypothesized to be an etiological factor in respiratory disease due to this exposure. In the present study, human lung epithelial cells in vitro responded to hexavalent chromium, manganese and nickel over a concentration range of 0.2-200 microM with a significant increase in intracellular phosphoprotein (a measure of stress response pathway activation). The mitogen-activated protein kinases ERK1/2, SAPK/JNK and p38 were activated via phosphorylation following 1-h exposures. Hexavalent chromium up-regulated p-38 phosphorylation 23-fold and SAPK/JNK phosphorylation 17-fold, with a comparatively modest 4-fold increase in ERK1/2 phosphorylation. Manganese caused a two- to four-fold increase in SAPK/JNK and ERK 1/2 phosphorylation, with no observed effects on p38 kinase. Nickel caused increased (two-fold) phosphorylation of ERK 1/2 only, and was not cytotoxic over the tested concentration range. The observed effects of welding fume metals on cellular signaling in lung epithelium demonstrate a potentially significant interplay between stress-response signaling (p38 and SAPK/JNK) and anti-apototic signaling (ERK 1/2) that is dependant on the specific metal or combination of metals involved. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Chromium Epidemiology Hazardous Substances Lung Lung Diseases Manganese Neoplasms Nickel Occupational Health Respiratory System Respiratory Tract Diseases Safety Welding

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• Keywords:
  Author Keywords: Welding Fumes Cancer Cell-cultures Cell-function Cell-growth Chromium-compounds Humans Lung-cancer Lung-disease Lung-disorders MAP Kinases Nickel-compounds Pulmonary-system-disorders Respiratory-system-disorders Toxic-effects Welders Welding-industry

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• ISSN:
  0378-4274
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Illinois ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  114-121
• Volume:
  167
• Issue:
  2
• NIOSHTIC Number:
  nn:20039117
• Citation:
  Toxicol Lett 2006 Dec; 167(2):114-121
• Email:
  dmt@uic.edu
• CAS Registry Number:
  Chromium(6+) (CAS RN 18540-29-9) ; Manganese (CAS RN 7439-96-5) ; Nickel (CAS RN 7440-02-0)
• Federal Fiscal Year:
  2007
• Performing Organization:
  University of Illinois at Chicago, School of Public Health
• Peer Reviewed:
  True
• Start Date:
  19930701
• Source Full Name:
  Toxicology Letters
• End Date:
  20030630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2e4b854f2e0eea7ae249d127bff545834dce34523517453b6250f21136a023b55f914ecef851b8b609b4d531c7ca3a8e7604e1ce460285f1af2c504670b0388a
• Download URL:
  https://stacks.cdc.gov/view/cdc/192591/cdc_192591_DS1.pdf
• File Type:
  [PDF - 470.82 KB ]