Cytotoxicity of chromium and manganese to lung epithelial cells in vitro

Details

• Personal Author:
  Pascal LE ; Tessier DM
• Description:
  Chromium, nickel and manganese are the predominant metals in welding fumes and are associated through epidemiological studies with an increased risk for developing occupational asthma due to welding activities. Here, we show that chromium(VI) and manganese, but not nickel, are cytotoxic to normal human lung epithelial cells in vitro (SAEC and BEAS-2B), at concentration ranges of 0.2-200 microM. The toxic effect was associated with increased levels of intracellular phosphoprotein and subsequent release of inflammatory cytokines IL-6 and IL-8, while no release of TNF-alpha was observed. Changes in intracellular phosphoprotein levels occurred at concentrations below the cytotoxic effect. IL-6 and IL-8 production increased up to 4.4-fold relative to controls. IL-6 and IL-8 are released from lung epithelium to recruit cells of the immune system to sites of tissue damage. Therefore, the observed effects of chromium(VI) and manganese in lung epithelial cells demonstrate a mechanism through which the toxicity of these metals to epithelial cells can result in recruitment of cells of the immune system. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Chromium Cytotoxins Lung Lung Diseases Manganese Metals Nickel Occupational Health Respiratory System Respiratory Tract Diseases Safety Welding

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• Keywords:
  Author Keywords: Lung Epithelium Chromium-compounds Cytotoxicity Fumes IL-6 IL-8 Lung-cells Manganese-compounds Metal-compounds Nickel-compounds Protein Phosphorylation Pulmonary-disorders Pulmonary-function Respiratory-system-disorders

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• ISSN:
  0378-4274
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Illinois ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  143-151
• Volume:
  147
• Issue:
  2
• NIOSHTIC Number:
  nn:20039021
• Citation:
  Toxicol Lett 2004 Mar; 147(2):143-151
• Contact Point Address:
  Daniel M. Tessier; Division of Environmental & Occupational Health Sciences, School of Public Health, University of Illinois Chicago, SPH/EOHS/MC922, 2121 West Taylor Street, Chicago, IL 60612
• Email:
  dmt@uic.edu
• CAS Registry Number:
  Chromium(6+) (CAS RN 18540-29-9) ; Manganese (CAS RN 7439-96-5) ; Nickel (CAS RN 7440-02-0)
• Federal Fiscal Year:
  2004
• Performing Organization:
  University of Illinois at Chicago
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Toxicology Letters
• End Date:
  20290630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:fcabc199136ed1735a579607e59ae73954a194dec8ff96fb732ae8ae04c7d42b0a2112e67dffc2f5fdb185ea56267d72b7a6de10f91258aaadaf7473ec203c97
• Download URL:
  https://stacks.cdc.gov/view/cdc/192518/cdc_192518_DS1.pdf
• File Type:
  [PDF - 243.02 KB ]