Transcriptomics evaluation of hexavalent chromium toxicity in human dermal fibroblasts

Details

• Personal Author:
  Chapman R ; Joseph P ; Kashon M ; Leonard, Stephanie ; Li S ; Sellamuthu R ; Umbright C
• Description:
  Significant exposure to hexavalent chromium, a metal with broad toxicity potential in humans, has been reported. In order to understand the mechanisms of dermal toxicity induced by hexavalent chromium, global gene expression profiling of human dermal fibroblasts exposed to potassium dichromate was performed. Microarray analysis of the gene expression profile in the fibroblasts treated with potassium dichromate identified significant differential expression of approximately 1,200 transcripts compared with the control cells. Functional categorization of the differentially expressed genes identified the enrichment of genes involved in several cellular processes, including apoptosis and oxidative stress, in the fibroblasts exposed to hexavalent chromium. Induction of apoptosis and oxidative stress in the dermal fibroblasts in response to their exposure to chromium was independently confirmed by additional experiments. The potassium dichromate-induced cytotoxicity, apoptosis, and oxidative stress were significantly blocked by the addition of ferrous sulfate, an agent known for its ability to reduce chromium to the insoluble and therefore impermeable trivalent form, to the cell culture medium. Taken together, our data provide insights into the potential mechanisms underlying the dermal toxicity of hexavalent chromium and provide experimental support for the proposed protective role of ferrous sulfate in hexavalent chromium-induced toxicity. [Description provided by NIOSH]
• Subjects:
  Carcinogens Cytotoxins Environmental Monitoring Genetics Hazardous Substances Metals Occupational Health Oxidants Poisoning Safety Skin Skin Absorption Skin Diseases Toxicology

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• Keywords:
  Apoptosis Author Keywords: Hexavalent Chromium Biohazards Biological-effects Cell-biology Cell-function Cellular-function Cellular-reactions Cytotoxic-effects Dermal Toxicity Exposure-assessment Exposure-levels Fibrogenesis Gene Expression Genetic-factors Humans Metal-compounds Metal-poisoning Metallic-compounds Metallic-poisoning Oxidation Oxidative-metabolism Oxidative Stress Physiological-effects Physiological-response Quantitative-analysis Skin-absorption Skin-diseases Skin-exposure Skin-irritants Skin-sensitivity

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• ISSN:
  2157-2518
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  2
• Issue:
  1
• NIOSHTIC Number:
  nn:20039099
• Citation:
  J Carcinog Mutagen 2011 Feb; 2(1):116
• Contact Point Address:
  Pius Joseph, Molecular Carcinogenesis Laboratory, National Institute for Occupational Safety and Health (NIOSH), MS 3014, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  pjoseph1@cdc.gov
• CAS Registry Number:
  Chromium(6+) (CAS RN 18540-29-9)
• Federal Fiscal Year:
  2011
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Carcinogenesis & Mutagenesis
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:554e540a01e3a1d61fd5c1449ef8055aa9dc88bcda1f31dc5c8e293bd8d38efca8a5aaf96f305c388a0c714480ca5eff2017f2b7577adf1a48bd98cbc1705d20
• Download URL:
  https://stacks.cdc.gov/view/cdc/192577/cdc_192577_DS1.pdf
• File Type:
  [PDF - 1.37 MB ]