Assessment of fibrogenic biomarkers induced by multi wall carbon nanotubes

Details

• Personal Author:
  Castranova, Vincent ; Mercer R ; Mishra A ; Rojanasakul Y ; Wang L
• Description:
  Multi Wall Carbon Nanotubes (MWCNT), a graphene based nanoparticle, possess unique physiochemical properties. Considered as a technological breakthrough, production of MWCNT is rapidly increasing worldwide but toxicity profile of the nanomaterials is not clearly understood. Among the adverse effects, CNT have been shown to induce (the development of unusual) interstitial lung fibrosis at physiologically relevant exposure (10ug/mouse); however, the underlying mechanism is not fully known. In this present study, we investigated important MWCNT-induced fibrogenic mediators using cultured lung cell systems. Human bronchial epithelial (BEAS-2B) cells, alveolar epithelial (A549) cells, and lung fibroblast (CRL1490) cells were treated with MWCNT. Viability of MWCNT-exposed cells was determined by cell counting and WST-1 assay. Fibrogenic mediators, including Fibroblast Growth Factor-2, Vascular Growth Factor, Transforming Growth Factor â1 (TGF-â1), and Platelet Derived Growth Factor-A, were analyzed using Western Blots and end point ELISA. Our results show that 1) MWCNT decreased cell viability of epithelial cells in a dose and time dependent manner, 2) MWCNT exposure induced secretion of fibrogenic mediators from lung epithelial cells and fibroblasts at physiologically relevant concentrations of 0.02 to 0.6 ug/cm2 and 3) MWCNT directly induced collagen production from lung fibroblasts. In conclusion, MWCNT induced fibrogenic mediators in cultured human lung epithelial cells and stimulated collagen production from fibroblasts. These data are consistent with in vivo observations. Therefore, the in-vitro cell culture systems can be used for mechanistic studies and screening tests for MWCNT and similar fibrogenic nanoparticles. [Description provided by NIOSH]
• Subjects:
  Cytotoxins Hazardous Substances Irritants Lung Lung Diseases Occupational Health Particulate Matter Respiratory Hypersensitivity Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Biological-effects Cell-biology Cell-damage Cell-growth Cellular-reactions Cytotoxic-effects Cytotoxicity Dose-response Exposure-levels Inhalation-studies Lung-disease Lung-disorders Lung-function Lung-irritants Nanotechnology Particulate-sampling-methods Physiological-effects Pulmonary-disorders Pulmonary-system-disorders Quantitative-analysis Respiratory-hypersensitivity Respiratory-irritants Respiratory-system-disorders Time-weighted-average-exposure Toxic-effects

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  District of Columbia ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  120
• NIOSHTIC Number:
  nn:20038431
• Citation:
  Toxicologist 2011 Mar; 120(Suppl 2):253
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 50th Annual Meeting and ToxExpo, March 6-10, 2011, Washington, DC
• Supplement:
  2
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b123f35bc6b105b296aef32f1c07e15ed35f2d5dbb3691f1b1dce67b68801c1da824bf64bb793d6dc3602152805de753be2413a0b132022d673445dcbfcc630e
• Download URL:
  https://stacks.cdc.gov/view/cdc/192144/cdc_192144_DS1.pdf
• File Type:
  [PDF - 1.10 MB ]