Vitamin E and C supplementation reduces oxidative stress, improves antioxidant enzymes and positive muscle work in chronically loaded muscles of aged rats

Details

• Personal Author:
  Alway SE ; Baker BA ; Cutlip, Robert G. ; Docherty M ; Dudash HJ ; Geronilla KB ; Haff GG ; Ryan MJ
• Description:
  Aging is associated with increased oxidative stress. Muscle levels of oxidative stress are further elevated with exercise. The purpose of this study was to determine if dietary antioxidant supplementation would improve muscle function and cellular markers of oxidative stress in response to chronic repetitive loading in aging. The dorsiflexors of the left limb of aged and young adult Fischer 344 Brown x Norway rats were loaded 3 times weekly for 4.5 weeks using 80 maximal stretch-shortening contractions per session. The contra-lateral limb served as the intra animal control. The rats were randomly assigned to a diet supplemented with Vitamin E and Vitamin C or normal non-supplemented rat chow. Biomarkers of oxidative stress were measured in the tibial's anterior muscle. Repetitive loading exercise increased maximal isometric force negative work and positive work in the dorsiflexors of young adult rats. Only positive work increased in the aged animals that were supplemented with Vitamin E and C. Markers of oxidative stress (H2O2 total GSH GSH/GSSG ratio malondialdehyde and 8-OHdG) increased in the tibialis anterior muscles from aged and young adult animals with repetitive loading, but Vitamin E and C supplements attenuated this increase MnSOD activity increased with supplementation in the young adult animals, CuZnSOD and catalase activity increased with supplementation in young adult and aged animals, and GPx activity increased with exercise in the non-supplemented young adult and aged animals. The increased levels of endogenous antioxidant enzymes after Vitamin E and C supplementation appear to be regulated by post transcriptional modifications that are affected differently by age exercise and supplementation. These data suggest that antioxidant supplementation improves indices of oxidative stress associated with repetitive loading exercise and aging and improves the positive work output of muscles in aged rodents. [Description provided by NIOSH]
• Subjects:
  Age Factors Aging Animals Antioxidants Biomarkers Diet Enzymes Laboratories Musculoskeletal System Occupational Health Safety

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• Keywords:
  Age-factors Animal-studies Antioxidant Author Keywords: Oxidative Stress Cellular-function Dietary-effects Enzyme-activity Exercise Laboratory-animals Muscle-function Muscles Nutrition Nutritional Supplementation Oxidative-processes Physical-exercise Physical-stress Vitamins

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• ISSN:
  0531-5565
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  45
• Issue:
  11
• NIOSHTIC Number:
  nn:20038064
• Citation:
  Exp Gerontol 2010 Nov; 45(11):882-895
• Contact Point Address:
  Stephen E. Alway, Division of Exercise Physiology, School of Medicine, Robert C. Byrd Health Sciences Center, West Virginia University, P.O. Box 9227, Morgantown, WV 26506-9227, USA
• Email:
  salway@hsc.wvu.edu
• Federal Fiscal Year:
  2011
• Peer Reviewed:
  True
• Source Full Name:
  Experimental Gerontology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:12e96760bf8370205ea2f89dfa1b1e6072e0547627336f653eba741a6d094676eed810eadac9e6c09c469b50e5ad5e31cbc21df3f00c7a6f49e44ee0e830f6f1
• Download URL:
  https://stacks.cdc.gov/view/cdc/191891/cdc_191891_DS1.pdf
• File Type:
  [PDF - 1.34 MB ]