Measurement of multiple drugs in urine, water, and on surfaces using fluorescence covalent microbead immunosorbent assay

Details

• Personal Author:
  Biagini, Raymond E. ; Robertson, Shirley ; Sammons D ; Smith J ; Snawder, John E.
• Description:
  There are a range of applications that require the measurement of multiple drugs such as urine analysis, drug determination in water, and screening for drug contamination on surfaces. Some of the procedures used such as enzyme-linked immunosorbent assay (ELISA) are simple but can only determine one drug at a time, and others such as GC-MS or LC-MS are complex, time-consuming, and expensive. In this study, fluorescence covalent microbead immunosorbent assay (FCMIA) was investigated as a simple method for the measurement of multiple drugs simultaneously in three matrices: diluted urine, water, and on surfaces. Five different drugs of abuse or their metabolites (methamphetamine, caffeine, benzoylecgonine (a metabolite of cocaine), tetrahydrocannabinol (THC), the active ingredient in marijuana, and oxycodone) were studied over the range 0-15 ng/ml. There was no measureable cross-reactivity among the drugs at the concentrations studied. Urine dilutions from 1/50 to 1/2.5 were studied and dilutions less than 1/20 had a significant effect on the methamphetamine assay but limited effects on the benzoylecgonine and oxycodone assays and almost no effect on the THC assay. For assays performed in 1/20 urine dilution, water, and diluted surface sampling buffer, least detectable doses (LDD) were 1 ng/ml or less for the drugs. Surfaces spiked with drugs were sampled with swabs wetted with surface sampling buffer and recoveries were linear over the range 0-100 ng/100 cm(2) surface loading for all drugs. FCMIA has potential to be used for the measurement of multiple drugs in the matrices studied. [Description provided by NIOSH]
• Subjects:
  Biological Assay Biopharmaceutics Energy Metabolism Immune System Occupational Health Safety Substance-Related Disorders Urine
• Keywords:
  Analytical-instruments Author Keywords: Multidrug Immunoassay Bioassays Drug-abuse Drugs Immunochemistry Measurement-equipment Metabolites Pharmaceuticals Sampling-equipment Sampling-methods Screening-methods Surface Sampling Urinalysis Urine Urine-chemistry Water

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• ISSN:
  1537-6516
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  20
• Issue:
  9
• NIOSHTIC Number:
  nn:20037876
• Citation:
  Toxicol Mech Methods 2010 Nov; 20(9):587-593
• Contact Point Address:
  Jerome Smith, National Institute for Occupational Safety and Health, Division of Applied Research and Technology, 4676 Columbia Parkway, Cincinnati, OH 45226-1998
• Email:
  jps3@cdc.gov
• CAS Registry Number:
  (-)-Oxycodone (CAS RN 76-42-6) ; Caffeine (CAS RN 58-08-2) ; Methamphetamine (CAS RN 537-46-2) ; Tetrahydrocannabinol (CAS RN 1972-08-3)
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Services ; Public Safety
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology Mechanisms and Methods
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:99c1b039f50dd6aff11d8cc7779f7f69fa95cbf407ac2e86366e7a7a99456701aef468e3bb586d80ddd956e118bd0ea7488da8271d7e07b9c4166569342b33b2
• Download URL:
  https://stacks.cdc.gov/view/cdc/191799/cdc_191799_DS1.pdf
• File Type:
  [PDF - 753.71 KB ]